Oscillations of nitric oxide concentration in the perturbed denitrification pathway of Paracoccus denitrificans
Jazyk angličtina Země Anglie, Velká Británie Médium print
Typ dokumentu časopisecké články
PubMed
1325776
PubMed Central
PMC1133025
DOI
10.1042/bj2860111
Knihovny.cz E-zdroje
- MeSH
- biologický transport MeSH
- buněčná membrána účinky léků fyziologie MeSH
- cytochromy skupiny c metabolismus MeSH
- ethylmaleimid farmakologie MeSH
- karbonylkyanid-m-chlorfenylhydrazon farmakologie MeSH
- kinetika MeSH
- membránové potenciály účinky léků MeSH
- nitritreduktasy izolace a purifikace metabolismus MeSH
- oxid dusnatý metabolismus MeSH
- oxidoreduktasy izolace a purifikace metabolismus MeSH
- Paracoccus denitrificans metabolismus fyziologie MeSH
- pentachlorfenol farmakologie MeSH
- rozpřahující látky farmakologie MeSH
- spotřeba kyslíku účinky léků MeSH
- transport elektronů účinky léků MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- cytochromy skupiny c MeSH
- ethylmaleimid MeSH
- karbonylkyanid-m-chlorfenylhydrazon MeSH
- nitric-oxide reductase MeSH Prohlížeč
- nitritreduktasy MeSH
- oxid dusnatý MeSH
- oxidoreduktasy MeSH
- pentachlorfenol MeSH
- rozpřahující látky MeSH
The metabolism of nitric oxide in Paracoccus denitrificans has been studied using a Clark-type electrode. The uncoupler carbonyl cyanide m-chlorophenylhydrazone (CCCP) and the SH reagent N-ethylmaleimide, both of which released nitric oxide from cells respiring nitrite, were found to be efficient inhibitors of nitric oxide reductase activity. Control experiments with another uncoupler, pentachlorophenol, showed that the inhibitory effect of CCCP was not the result of a decrease in membrane potential. The denitrification pathway in cells with partly inhibited nitric oxide reductase, or in a reconstituted system containing purified nitric reductase and membrane vesicles, exhibited marked sustained oscillations of nitric oxide concentration. The occurrence of the oscillations was strictly dependent on the initial concentration of nitrite. The observed oscillatory kinetics is considered to reflect two regulatory signals destabilizing the denitrification pathway, namely the inhibition of nitric oxide reductase by nitric oxide and/or by nitrite.
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