Oscillations of nitric oxide concentration in the perturbed denitrification pathway of Paracoccus denitrificans
Language English Country England, Great Britain Media print
Document type Journal Article
PubMed
1325776
PubMed Central
PMC1133025
DOI
10.1042/bj2860111
Knihovny.cz E-resources
- MeSH
- Biological Transport MeSH
- Cell Membrane drug effects physiology MeSH
- Cytochrome c Group metabolism MeSH
- Ethylmaleimide pharmacology MeSH
- Carbonyl Cyanide m-Chlorophenyl Hydrazone pharmacology MeSH
- Kinetics MeSH
- Membrane Potentials drug effects MeSH
- Nitrite Reductases isolation & purification metabolism MeSH
- Nitric Oxide metabolism MeSH
- Oxidoreductases isolation & purification metabolism MeSH
- Paracoccus denitrificans metabolism physiology MeSH
- Pentachlorophenol pharmacology MeSH
- Uncoupling Agents pharmacology MeSH
- Oxygen Consumption drug effects MeSH
- Electron Transport drug effects MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Cytochrome c Group MeSH
- Ethylmaleimide MeSH
- Carbonyl Cyanide m-Chlorophenyl Hydrazone MeSH
- nitric-oxide reductase MeSH Browser
- Nitrite Reductases MeSH
- Nitric Oxide MeSH
- Oxidoreductases MeSH
- Pentachlorophenol MeSH
- Uncoupling Agents MeSH
The metabolism of nitric oxide in Paracoccus denitrificans has been studied using a Clark-type electrode. The uncoupler carbonyl cyanide m-chlorophenylhydrazone (CCCP) and the SH reagent N-ethylmaleimide, both of which released nitric oxide from cells respiring nitrite, were found to be efficient inhibitors of nitric oxide reductase activity. Control experiments with another uncoupler, pentachlorophenol, showed that the inhibitory effect of CCCP was not the result of a decrease in membrane potential. The denitrification pathway in cells with partly inhibited nitric oxide reductase, or in a reconstituted system containing purified nitric reductase and membrane vesicles, exhibited marked sustained oscillations of nitric oxide concentration. The occurrence of the oscillations was strictly dependent on the initial concentration of nitrite. The observed oscillatory kinetics is considered to reflect two regulatory signals destabilizing the denitrification pathway, namely the inhibition of nitric oxide reductase by nitric oxide and/or by nitrite.
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Arch Microbiol. 1991;156(1):62-9 PubMed
J Biol Chem. 1991 Jun 15;266(17):10899-905 PubMed
Biochim Biophys Acta. 1990 May 15;1017(1):57-62 PubMed
Biochem J. 1990 Jul 15;269(2):423-9 PubMed
J Basic Microbiol. 1990;30(7):515-22 PubMed
Gen Physiol Biophys. 1990 Oct;9(5):501-17 PubMed
Eur J Biochem. 1983 Feb 1;130(2):359-64 PubMed
Biochim Biophys Acta. 1983 May 27;723(2):231-9 PubMed
Appl Environ Microbiol. 1981 Dec;42(6):1074-84 PubMed
Eur J Biochem. 1983 Oct 17;136(1):135-40 PubMed
Biochim Biophys Acta. 1981 Jan 8;640(1):341-51 PubMed
J Biol Chem. 1990 Jan 15;265(2):889-95 PubMed
J Biol Chem. 1990 Jul 15;265(20):11535-8 PubMed
J Biol Chem. 1989 May 5;264(13):7556-63 PubMed
Biochem J. 1987 Sep 15;246(3):779-82 PubMed
Eur J Biochem. 1989 Feb 15;179(3):683-92 PubMed
Biochim Biophys Acta. 1987 Feb 25;911(3):334-40 PubMed
Arch Biochem Biophys. 1974 Jan;160(1):215-22 PubMed
Biochem Biophys Res Commun. 1984 Sep 28;123(3):966-72 PubMed
Biochim Biophys Acta. 1979 Jul 4;585(3):462-76 PubMed
J Bioenerg Biomembr. 1991 Apr;23(2):163-85 PubMed
