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In vitro modeling of amyotrophic lateral sclerosis with induced pluripotent stem cell technology-derived cell line ORIONi002-A
J. Strnadel, HM. Dumortier, D. Hajduchova, R. Zahumenska, V. Nosal, M. Smolar, J. Marcinek, M. Kalman, S. Mersakova, D. Brany, S. Juhas, J. Juhasova, H. Studenovska, B. Mitruskova, S. Suroviakova, S. Novakova, H. Skovierova, E. Kurca, R. Pecova,...
Stem cell research.
2022 ;
63 (-) :
102870.
[pub] 20220713
Language English Country England, Great Britain
Document type Journal Article, Research Support, Non-U.S. Gov't
Persistent link
https://www.medvik.cz/link/bmc22025120
Online
Full text
NLK
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- MeSH
- Amyotrophic Lateral Sclerosis * pathology MeSH
- Cell Differentiation MeSH
- Cell Line MeSH
- Fibroblasts metabolism MeSH
- Induced Pluripotent Stem Cells * metabolism MeSH
- Humans MeSH
- Technology MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
We present here a new iPS cell line for modeling sporadic form of ALS. Cell line was generated by reprogramming skin fibroblasts isolated with explant culture technology from skin biopsy, donated by ALS patient. For reprogramming, polycistronic self-replicating RNA vector was used and derived iPS cells were characterized by immunocytochemistry and FACS (pluripotent factors expression), karyotyping, STR fingerprinting analysis and in vitro differentiation assay. New cell line showed normal (46, XY) karyotype and differentiated in vitro into cells from three germ layers. STR analysis proved the origin and originality of the cell line.
References provided by Crossref.org
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