• This record comes from PubMed

Isolation of auxotrophic mutants of the methylotrophic yeast Candida boidinii and determination of its ploidy

. 1992 Oct ; 62 (3) : 167-71.

Language English Country Netherlands Media print

Document type Journal Article

Persistent link   https://www.medvik.cz/link/pmid01416913

Auxotrophic mutations in the methylotrophic yeast strain Candida boidinii 11Bh were induced by different mutagens and their combinations (nitrosoguanidine, UV light, HNO2+UV). Majority of the mutants obtained carried defects in histidine, arginine, proline and/or adenine biosynthetic pathways. His- mutants were distributed into four complementation groups using the protoplasts fusion technique. Ploidy determination of Candida boidinii 11Bh was performed by measuring its DNA content and by following its survival after chemical mutagens treatment. The DNA content of this strain was found to be similar to that of a Saccharomyces cerevisiae diploid strain. Also the kinetics of survival of Candida boidinii cells indicate that Candida boidinii 11Bh is a diploid.

See more in PubMed

Annu Rev Genet. 1971;5:163-218 PubMed

Nature. 1966 Jul 9;211(5045):206-7 PubMed

Mutat Res. 1965 Aug;2(4):328-31 PubMed

Folia Microbiol (Praha). 1974;19(4):249-56 PubMed

Biotechnology (N Y). 1991 May;9(5):455-60 PubMed

Mol Cell Biol. 1985 Dec;5(12):3376-85 PubMed

J Mol Biol. 1973 Sep 5;79(1):65-82 PubMed

Mol Cell Biol. 1986 Jan;6(1):142-9 PubMed

Adv Microb Physiol. 1983;24:1-82 PubMed

Eur J Biochem. 1973 Jul 2;36(1):250-6 PubMed

Anal Biochem. 1970 Nov;38(1):190-201 PubMed

Eur J Biochem. 1976 Feb 2;62(1):151-60 PubMed

J Gen Microbiol. 1986 Feb;132(2):263-8 PubMed

Find record

Citation metrics

Logged in users only

Archiving options