New trends in the use of Al(OH)3-conjugated endotoxins and their subunits from the S- and R-forms of Shigella dysenteriae serovar 1 for model vaccination purposes
Jazyk angličtina Země Nizozemsko Médium print
Typ dokumentu časopisecké články, Research Support, U.S. Gov't, Non-P.H.S., Research Support, U.S. Gov't, P.H.S.
Grantová podpora
HL 31035
NHLBI NIH HHS - United States
PubMed
1905447
DOI
10.1016/0264-410x(91)90265-8
PII: 0264-410X(91)90265-8
Knihovny.cz E-zdroje
- MeSH
- bacilární dyzentérie prevence a kontrola MeSH
- bakteriální vakcíny izolace a purifikace toxicita MeSH
- endotoxiny izolace a purifikace toxicita MeSH
- hydroxid hlinitý MeSH
- imunodifuze MeSH
- králíci MeSH
- LD50 MeSH
- Limulus test MeSH
- myši inbrední ICR MeSH
- myši MeSH
- pyrogeny MeSH
- Shigella dysenteriae klasifikace imunologie MeSH
- Shwartzmanův fenomén MeSH
- zvířata MeSH
- Check Tag
- králíci MeSH
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Research Support, U.S. Gov't, Non-P.H.S. MeSH
- Research Support, U.S. Gov't, P.H.S. MeSH
- Názvy látek
- bakteriální vakcíny MeSH
- endotoxiny MeSH
- hydroxid hlinitý MeSH
- pyrogeny MeSH
The lipopolysaccharides, lipopolysaccharide-protein complexes and their lipids A, isolated from Shigella dysenteriae 1, exhibited lethal toxicity (LD50 300-400 micrograms per mouse), pyrogenicity (0.01-1.0 microgram), activity in the Limulus test (10(-3)-10(-12) mg ml-1) and produced a positive local Shwartzman reaction. It was found that these substances bound to Al(OH)3 were capable of successfully protecting mice from challenge with both homologous and heterologous infections (up to 90% of mice survived a challenge with LD100), whereas immunization with substances with no biological carrier exhibited very low or zero protective effects. Furthermore, the positive cross-reactivity of lipids A in the agar immunodiffusion test confirms the wide applicability for vaccination purposes.
Citace poskytuje Crossref.org
