Transitions of poly(dI-dC), poly(dI-methyl5dC) and poly(dI-bromo5dC) among and within the B-, Z-, A- and X-DNA families of conformations
Language English Country Great Britain, England Media print
Document type Journal Article
PubMed
2041775
PubMed Central
PMC329441
DOI
10.1093/nar/19.9.2343
Knihovny.cz E-resources
- MeSH
- Magnesium Chloride MeSH
- Sodium Chloride MeSH
- Circular Dichroism MeSH
- DNA chemistry MeSH
- Isomerism MeSH
- Nucleic Acid Conformation MeSH
- Polydeoxyribonucleotides chemistry MeSH
- Solutions MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Magnesium Chloride MeSH
- Sodium Chloride MeSH
- DNA MeSH
- poly d(I-C) MeSH Browser
- polydeoxyinosinic-polydeoxy-(5-bromocytidylic)acid MeSH Browser
- polydeoxyinosinic-polydeoxy-(5-methylcytidylic)acid MeSH Browser
- Polydeoxyribonucleotides MeSH
- Solutions MeSH
It is shown, using circular dichroism spectroscopy, that poly(dI-dC) is capable to isomerize into both Z-DNA and A-DNA in concentrated NaCl + NiCl2 and trifluoroethanol solutions, respectively. This polynucleotide also undergoes a cooperative, two-state transition in ethanol into a structure which most probably is a canonical B-DNA. This implies that the conformation of poly(dI-dC) is unusual in low-salt aqueous solution. The canonical B-DNA is also adopted by poly(dI-methyl5dC) in trifluoroethanol while this polynucleotide adopts Z-DNA not only in NaCl + NiCl2 but also in the presence of MgCl2. Poly(dI-methyl5dC) partially adopts X-DNA in concentrated CsF and mainly ethanolic solutions. Poly(dI-bromo5dC) isomerizes into Z-DNA not only in concentrated NaCl even in the absence of NiCl2 but also in concentrated MgCl2. This polynucleotide transforms between two distinct variants of Z-DNA in ethanol or trifluoroethanol solutions.
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