Ducks: a new experimental host system for studying persistent infection with avian leukaemia retroviruses
Jazyk angličtina Země Anglie, Velká Británie Médium print
Typ dokumentu časopisecké články
- MeSH
- DNA virů genetika izolace a purifikace MeSH
- hybridizace nukleových kyselin MeSH
- kachny mikrobiologie MeSH
- molekulová hmotnost MeSH
- neutralizační testy MeSH
- protilátky virové analýza MeSH
- ptačí leukóza imunologie patofyziologie MeSH
- restrikční mapování MeSH
- viremie patofyziologie MeSH
- virus ptačí leukózy genetika patogenita MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- DNA virů MeSH
- protilátky virové MeSH
Long-term persistence of the avian leukosis virus (ALV), the transformation-defective mutant of Prague strain Rous sarcoma virus subgroup C (td PR-C) was established in heterologous duck hosts after infection in mid-embryogenesis. Transient viraemia was observed for about 4 weeks after hatching and was lost in most of the infected ducks by about 6 months. Loss of viraemia was accompanied by the increasing synthesis of virus-neutralizing antibodies. In spite of strong virus-neutralizing antibodies, virus was detected by the cocultivation assay in duck tissues throughout the observation period up to 5 years. In the viraemic phase of infection, we found integrated proviruses in various tissues, preferentially in stomach muscle tissue and in the thymus. The long-term persistence of virus was frequently accompanied by liver necrosis and neoplastic diseases. Injection of td PR-C virus into early embryos resulted in more pronounced infection accompanied by an increased copy number of viral DNA per cell, high mortality and remarkable atrophy of thymus tissue in infected ducklings.
Citace poskytuje Crossref.org
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