A monoclonal antibody, VI-01, to vimentin and desmin has been isolated from a fusion of mouse myeloma cells with immune spleen cells. The antibody appeared specific for vimentin of various species and smooth muscle desmin and did not recognize other intermediate filament proteins when cell lines and tissue sections were assayed in immunofluorescence and cell lysates in immunoblotting. The binding test with purified vimentin and desmin confirmed these results. The antibody can differentiate carcinomas from sarcomas in human diagnostic surgical pathology.

Institute of Molecular Genetics Czechoslovak Academy of Sciences Praha

Regulation of microtubule nucleation in mouse bone marrow-derived mast cells by ARF GTPase-activating protein GIT2

Glycol porphyrin derivatives and temoporfin elicit resistance to photodynamic therapy by different mechanisms

Exposure of beta-tubulin regions defined by antibodies on an Arabidopsis thaliana microtubule protofilament model and in the cells

Pontin is localized in nucleolar fibrillar centers

Association of brain gamma-tubulins with alpha beta-tubulin dimers

Post-translational modifications and multiple tubulin isoforms in Nicotiana tabacum L. cells

Expression of phosphorylated high molecular weight neurofilament protein (NF-H) and vimentin in human developing dorsal root ganglia and spinal cord

Expression of vimentin and glial fibrillary acidic protein in human developing spinal cord

Immunocytochemistry and heterogeneity of rat brain vimentin