Supercoil-induced Z-DNA formation within 5'-end of chicken myb proto-oncogene
Language English Country United States Media print
Document type Journal Article
PubMed
2645867
DOI
10.1016/0006-291x(89)92783-6
PII: 0006-291X(89)92783-6
Knihovny.cz E-resources
- MeSH
- Alkylation MeSH
- Diethyl Pyrocarbonate MeSH
- Electrophoresis, Agar Gel MeSH
- Introns MeSH
- Cloning, Molecular MeSH
- Codon MeSH
- Nucleic Acid Conformation * MeSH
- Chickens MeSH
- Molecular Sequence Data MeSH
- Osmium Tetroxide MeSH
- Plasmids MeSH
- Proto-Oncogene Proteins c-myb MeSH
- Proto-Oncogene Proteins genetics MeSH
- Base Sequence MeSH
- DNA, Superhelical * MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Diethyl Pyrocarbonate MeSH
- Codon MeSH
- Osmium Tetroxide MeSH
- Proto-Oncogene Proteins c-myb MeSH
- Proto-Oncogene Proteins MeSH
- DNA, Superhelical * MeSH
We have analyzed the recently sequenced and characterized 2.9 kb fragment derived from the 5'-end of chicken myb proto-oncogene with respect to structural perturbations induced by DNA supercoiling. Within the first intron a 50 bp sequence stretch was localized, starting approximately 450 nucleotides downstream from putative ATG initiation codon, which forms a non-B-DNA structure. Fine mapping with structural probes revealed the three adjacent regions with imperfect purine-pyrimidine alternation creating together relatively long Z-forming tract, parts of which may undergo a B-Z DNA transition at different superhelical densities.
References provided by Crossref.org
Transcription of the chicken myb proto-oncogene starts within a CpG island
