A thy- tetracycline-resistant mutant of Streptomyces granaticolor was prepared by mutagenesis of the parental strain ETH 7437. The mutant exhibits a different morphology and an overproduction of granaticins. The ability to form an aerial mycelium and spores has been lost. The mutant cells have a round or an atypical shape and a thick cell wall, the membraneous system is enlarged by numerous mesosomes. Division septa are formed rarely. The mutant is more sensitive to both low and high temperature than the parental strain. The altered features are stably maintained for many generations. Ribosomal proteins of the mutant do not differ substantially from those of the original strain indicating that the mutant phenotype is not due to an alteration at the translational level.

Czechoslovak Academy of Sciences Institute of Microbiology Prague

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Characterization of an asporogenous mutant of Streptomyces aureofaciens with increased resistance to several aminoglycoside antibiotics

Effect of granaticin on the activity of DNA-directed RNA polymerase from Streptomyces granaticolor