Intracellular ATP in a glucosephosphate isomerase mutant of Saccharomyces cerevisiae
Language English Country United States Media print
Document type Journal Article
PubMed
3519387
DOI
10.1007/bf02926828
Knihovny.cz E-resources
- MeSH
- Adenosine Triphosphate metabolism MeSH
- Phosphorylation MeSH
- Glucose-6-Phosphate Isomerase metabolism MeSH
- Glucose metabolism MeSH
- Carbohydrate Metabolism MeSH
- Mutation MeSH
- Saccharomyces cerevisiae enzymology genetics metabolism MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Adenosine Triphosphate MeSH
- Glucose-6-Phosphate Isomerase MeSH
- Glucose MeSH
The degree of ATP depletion caused by glucose in a glucosephosphate isomerase-deficient strain of Saccharomyces cerevisiae was determined. Even in the presence of a sugar normally fermentable by the mutant, the addition of glucose can decrease the intracellular ATP, depending on the competition of the sugars for transport and subsequent phosphorylation. For both parent and mutant cells, a correlation exists between the calculated velocity of ATP formation or ATP consumption during the utilization of different concentrations of sugars and the experimental intracellular ATP level. For initially resting yeast cells, a rate increase of 35 mumol per min per g ATP was calculated to increase the intracellular level of this nucleotide by 1 mumol per g cell mass.
See more in PubMed
J Bacteriol. 1979 Jul;139(1):152-60 PubMed
J Bacteriol. 1977 May;130(2):746-9 PubMed
Eur J Biochem. 1980 Apr;105(2):419-24 PubMed
J Bacteriol. 1971 Sep;107(3):759-69 PubMed
FEBS Lett. 1983 May 30;156(1):11-4 PubMed
Biochim Biophys Acta. 1976 Sep 13;440(3):661-74 PubMed
Anal Biochem. 1982 Jun;123(1):201-4 PubMed
Biochimie. 1973;55(2):205-11 PubMed
Eur J Biochem. 1984 Jan 16;138(2):407-11 PubMed
Folia Microbiol (Praha). 1967;12(2):121-31 PubMed
Mol Cell Biochem. 1976 Nov 30;13(2):73-8 PubMed
