Acidification power, defined as the sum of the spontaneous pH change determined after suspending yeast cells in water and the substrate-induced pH change after addition of glucose to the resulting suspension, reflects the level of cellular energy sources. Its use as an indicator of metabolic state of the cells was tested during a 120-h aerobic starvation. Its changes coincided with changes in cell viability, initial rate of endogenous oxygen consumption rate, cell ATP, extra- and intracellular buffering capacity, and the ability of cell-free extract to produce acidity by glucose fermentation. It was used as a sensitive marker of metabolic changes occurring during starvation, on treatment with glycolytic and respiratory inhibitors, and at elevated temperature.

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Biochem Biophys Res Commun. 1976 Sep 7;72(1):121-30 PubMed

Biochim Biophys Acta. 1974 Mar 15;339(2):274-84 PubMed

Biochim Biophys Acta. 1958 Feb;27(2):267-76 PubMed

Biochim Biophys Acta. 1981 May 20;643(3):583-92 PubMed

J Bacteriol. 1978 Feb;133(2):780-5 PubMed

Biochim Biophys Acta. 1958 Feb;27(2):255-66 PubMed

J Bacteriol. 1978 Feb;133(2):867-77 PubMed

Z Klin Chem Klin Biochem. 1967 Nov;5(6):299-304 PubMed

Adv Microb Physiol. 1980;21:267-338 PubMed

Biochim Biophys Acta. 1981 May 20;643(3):572-82 PubMed

J Biol Chem. 1951 Nov;193(1):265-75 PubMed

Biochim Biophys Acta. 1979 Apr 4;552(2):322-30 PubMed

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