Immunogenicity of N-(2-hydroxypropyl)-methacrylamide copolymers--potential hapten or drug carriers
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu časopisecké články
PubMed
6873772
DOI
10.1007/bf02884085
Knihovny.cz E-zdroje
- MeSH
- akrylamidy imunologie MeSH
- farmaceutická vehikula imunologie MeSH
- H-2 antigeny genetika MeSH
- hapteny imunologie MeSH
- imunizace MeSH
- inbrední kmeny myší imunologie MeSH
- kyseliny polymethakrylové imunologie MeSH
- myši inbrední C57BL imunologie MeSH
- myši MeSH
- protilátky analýza MeSH
- tvorba protilátek MeSH
- zvířata MeSH
- Check Tag
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- akrylamidy MeSH
- farmaceutická vehikula MeSH
- H-2 antigeny MeSH
- hapteny MeSH
- kyseliny polymethakrylové MeSH
- N-(2-hydroxypropyl)methacrylamide MeSH Prohlížeč
- protilátky MeSH
After repeated i.p. immunizations of mice with 10 micrograms of homopolymer poly (HPMA) no antibodies were detected by the ELISA test. Immunization with copolymer P-Acap-Leu-HMDA leads to a weak antibody response, while immunization with a copolymer with some side chains modified with ARS or FITC groups (P-Acap-Leu-HMDA-ARS or P-Acap-Leu-HMDA-FITC) leads to a significant antibody response detectable by PFC, ELISA and haemagglutination tests. Most of these antibodies are aimed against the modifying haptenic group, a smaller amount against side oligopeptide sequences of the carrier. Intensity of the antibody response depends on: 1) the antigen dose--the optimal dose was 10 micrograms: both the higher (100 micrograms) and the lower doses (1 and 0.1 micrograms) induced considerably lower antibody responses; 2) molar mass of the immunizing fractions--fractions of high molar mass induced up to five times higher responses than those of a low molar mass; 3) the bound haptenic group--the ARS-copolymers induced ten times lower response than the FITC-copolymers. We detected no difference between capacities of the H-2a, H-2b and H-2d haplotypes to react with anti-ARS antibodies after immunization with P-Acap-Leu-HMDA-ARS.
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