A new method was successfully used in collecting the mature larvae of the cattle warble fly by means of woven wire flooring. In laboratory colonies the influence of temperature on pupal development, percentage of hatched adults and sex ratio, weight of pupae and adults, its changes during their life cycle, copulation and survival of adults were studied. Field experiments involved studies on the influence of humidity on the survival of pupae, percentage of hatched adults, duration of the pupal stage in the spring and summer periods. Stability of the total sum of temperatures above the temperature threshold necessary for the development of adult in pupa, facilitating to determine the term of hatching, was demonstrated. Laboratory rearing made it possible to obtain gravid females of the cattle warble fly for experimental purposes.