A method for preparing M13 or pUC libraries for sequencing DNA with high G + C or A + T contents
Jazyk angličtina Země Nizozemsko Médium print
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
7489903
DOI
10.1016/0378-1119(95)00534-d
PII: 037811199500534D
Knihovny.cz E-zdroje
- MeSH
- genová knihovna MeSH
- sekvenční analýza DNA metody MeSH
- zastoupení bazí MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
A simple method is described to generate M13 or pUC libraries from DNA with a very high G + C or A + T content. The G + C-rich DNA is partially digested with HinPI or HpaII restriction enzymes and cloned into the vector linearized in its multiple cloning site with AccI. The A + T-rich DNA is partially digested with TspI and cloned into the EcoRI-linearized vector. These libraries are suitable for large-scale DNA sequencing.
Citace poskytuje Crossref.org
Complete genome sequence and analysis of the Streptomyces aureofaciens phage mu1/6
WD-repeat protein encoding genes among prokaryotes of the Streptomyces genus
Sequence of a 189-kb segment of the chromosome of Rhodobacter capsulatus SB1003
