A method for preparing M13 or pUC libraries for sequencing DNA with high G + C or A + T contents
Language English Country Netherlands Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
7489903
DOI
10.1016/0378-1119(95)00534-d
PII: 037811199500534D
Knihovny.cz E-resources
- MeSH
- Gene Library MeSH
- Sequence Analysis, DNA methods MeSH
- Base Composition MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
A simple method is described to generate M13 or pUC libraries from DNA with a very high G + C or A + T content. The G + C-rich DNA is partially digested with HinPI or HpaII restriction enzymes and cloned into the vector linearized in its multiple cloning site with AccI. The A + T-rich DNA is partially digested with TspI and cloned into the EcoRI-linearized vector. These libraries are suitable for large-scale DNA sequencing.
References provided by Crossref.org
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