Serodiagnosis of cercarial dermatitis with antigens of Trichobilharzia szidati and Schistosoma mansoni
Jazyk angličtina Země Česko Médium print
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
7812239
Knihovny.cz E-zdroje
- MeSH
- antigeny helmintové * krev imunologie MeSH
- časové faktory MeSH
- dermatitida diagnóza MeSH
- ELISA MeSH
- fluorescenční protilátková technika MeSH
- lidé MeSH
- myši MeSH
- parazitární onemocnění kůže diagnóza MeSH
- protilátky helmintové analýza krev MeSH
- Schistosoma mansoni imunologie MeSH
- Schistosomatidae imunologie MeSH
- sérologické testy MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Geografické názvy
- Československo MeSH
- Názvy látek
- antigeny helmintové * MeSH
- protilátky helmintové MeSH
In patients with parasitologically revealed dermatitis caused by cercariae of avian schistosomes (Trichobilhariza szidati) diagnostic indirect immunofluorescence technique (IFAT) was employed for the detection of antibodies. The efficacy of antigens prepared from cercariae of T. szidati and Schistosoma mansoni was tested in serodiagnosis. The results have shown that antigen of T. szidati is more reactive with the sera of patients than that of S. mansoni: the antibodies were detected already 3 days after penetration of cercariae, contrary to 10 days after penetration of avian schistosomes when antigen of S. mansoni was used. The results were confirmed with enzyme-linked immunosorbent assay (ELISA) and IFAT techniques in SPF mice (Mus musculus) experimentally infected with cercariae of T. szidati and S. mansoni or with fractions isolated from cercariae of T. szidati.
