Serodiagnosis of cercarial dermatitis with antigens of Trichobilharzia szidati and Schistosoma mansoni
Language English Country Czech Republic Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
7812239
Knihovny.cz E-resources
- MeSH
- Antigens, Helminth * blood immunology MeSH
- Time Factors MeSH
- Dermatitis diagnosis MeSH
- Enzyme-Linked Immunosorbent Assay MeSH
- Fluorescent Antibody Technique MeSH
- Humans MeSH
- Mice MeSH
- Skin Diseases, Parasitic diagnosis MeSH
- Antibodies, Helminth analysis blood MeSH
- Schistosoma mansoni immunology MeSH
- Schistosomatidae immunology MeSH
- Serologic Tests MeSH
- Animals MeSH
- Check Tag
- Humans MeSH
- Mice MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Geographicals
- Czechoslovakia MeSH
- Names of Substances
- Antigens, Helminth * MeSH
- Antibodies, Helminth MeSH
In patients with parasitologically revealed dermatitis caused by cercariae of avian schistosomes (Trichobilhariza szidati) diagnostic indirect immunofluorescence technique (IFAT) was employed for the detection of antibodies. The efficacy of antigens prepared from cercariae of T. szidati and Schistosoma mansoni was tested in serodiagnosis. The results have shown that antigen of T. szidati is more reactive with the sera of patients than that of S. mansoni: the antibodies were detected already 3 days after penetration of cercariae, contrary to 10 days after penetration of avian schistosomes when antigen of S. mansoni was used. The results were confirmed with enzyme-linked immunosorbent assay (ELISA) and IFAT techniques in SPF mice (Mus musculus) experimentally infected with cercariae of T. szidati and S. mansoni or with fractions isolated from cercariae of T. szidati.
