Factors affecting the susceptibility of sensitive yeast cells to killer toxin K1
Language English Country United States Media print
Document type Journal Article
PubMed
8150399
DOI
10.1007/bf02814408
Knihovny.cz E-resources
- MeSH
- Drug Resistance, Microbial MeSH
- Potassium Chloride pharmacology MeSH
- Energy Metabolism MeSH
- Magnesium pharmacology MeSH
- Killer Factors, Yeast MeSH
- Hydrogen-Ion Concentration MeSH
- Mycotoxins toxicity MeSH
- Saccharomyces cerevisiae Proteins MeSH
- Saccharomyces cerevisiae drug effects metabolism MeSH
- Calcium pharmacology MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Potassium Chloride MeSH
- Magnesium MeSH
- Killer Factors, Yeast MeSH
- Mycotoxins MeSH
- Saccharomyces cerevisiae Proteins MeSH
- Calcium MeSH
Optimum conditions for action of the killer toxin K1 on sensitive strain S. cerevisiae S6 were established. Maximum killing was reached in a very narrow pH range of 4.5-4.6. Maximum susceptibility to toxin was displayed by highly energized fresh cells from the early exponential phase in the presence of an external energy source (at least 200 mmol/L glucose). Further, maintenance of maximum membrane potential was necessary for killer action, as documented by decreasing toxin activity in the presence of increasing concentrations of KCl. The killing was strongly stimulated in the presence of millimolar concentrations of Ca2+ and Mg2+.
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Yeast killer toxin K1 and its exploitation in genetic manipulations
