Factors affecting the susceptibility of sensitive yeast cells to killer toxin K1
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu časopisecké články
PubMed
8150399
DOI
10.1007/bf02814408
Knihovny.cz E-zdroje
- MeSH
- antibiotická rezistence MeSH
- chlorid draselný farmakologie MeSH
- energetický metabolismus MeSH
- hořčík farmakologie MeSH
- killer faktory kvasinek MeSH
- koncentrace vodíkových iontů MeSH
- mykotoxiny toxicita MeSH
- Saccharomyces cerevisiae - proteiny MeSH
- Saccharomyces cerevisiae účinky léků metabolismus MeSH
- vápník farmakologie MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- chlorid draselný MeSH
- hořčík MeSH
- killer faktory kvasinek MeSH
- mykotoxiny MeSH
- Saccharomyces cerevisiae - proteiny MeSH
- vápník MeSH
Optimum conditions for action of the killer toxin K1 on sensitive strain S. cerevisiae S6 were established. Maximum killing was reached in a very narrow pH range of 4.5-4.6. Maximum susceptibility to toxin was displayed by highly energized fresh cells from the early exponential phase in the presence of an external energy source (at least 200 mmol/L glucose). Further, maintenance of maximum membrane potential was necessary for killer action, as documented by decreasing toxin activity in the presence of increasing concentrations of KCl. The killing was strongly stimulated in the presence of millimolar concentrations of Ca2+ and Mg2+.
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Yeast killer toxin K1 and its exploitation in genetic manipulations
