Antibody response to a synthetic peptide derived from the human papillomavirus type 6/11 L2 protein in recurrent respiratory papillomatosis: correlation between Southern blot hybridization, polymerase chain reaction, and serology
Language English Country United States Media print
Document type Comparative Study, Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.
Grant support
5P30 CA-13330
NCI NIH HHS - United States
PubMed
8308521
DOI
10.1002/jmv.1890420111
Knihovny.cz E-resources
- MeSH
- Child MeSH
- DNA, Viral analysis MeSH
- Adult MeSH
- Enzyme-Linked Immunosorbent Assay MeSH
- Capsid immunology MeSH
- Middle Aged MeSH
- Humans MeSH
- Neoplasm Recurrence, Local MeSH
- Adolescent MeSH
- Molecular Sequence Data MeSH
- Respiratory Tract Neoplasms immunology virology MeSH
- Oncogene Proteins, Viral immunology MeSH
- Papillomaviridae immunology MeSH
- Papilloma immunology virology MeSH
- Peptide Fragments immunology MeSH
- Polymerase Chain Reaction MeSH
- Child, Preschool MeSH
- Antibodies, Viral analysis MeSH
- Amino Acid Sequence MeSH
- Aged, 80 and over MeSH
- Aged MeSH
- Blotting, Southern MeSH
- Capsid Proteins * MeSH
- Check Tag
- Child MeSH
- Adult MeSH
- Middle Aged MeSH
- Humans MeSH
- Adolescent MeSH
- Male MeSH
- Child, Preschool MeSH
- Aged, 80 and over MeSH
- Aged MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Research Support, U.S. Gov't, P.H.S. MeSH
- Comparative Study MeSH
- Names of Substances
- DNA, Viral MeSH
- L2 oncogene viral capsid protein, Human papillomavirus type 6 MeSH Browser
- Oncogene Proteins, Viral MeSH
- Peptide Fragments MeSH
- Antibodies, Viral MeSH
- Capsid Proteins * MeSH
Recurrent respiratory papillomatosis (RRP) is the most common benign tumour of the larynx, affecting both children and adults. We present a series of 25 patients, including 10 cases of juvenile multiple, 8 cases of adult solitary, and 7 cases of adult multiple RRP. Biopsy tissue from each patient was screened by Southern blot hybridization and polymerase chain reaction for the presence of human papillomavirus (HPV) DNA. Sera from patients and age- and sex-matched controls were tested for the presence of HPV-specific antibodies using a synthetic peptide derived from the minor capsid protein (L2) of HPV 6/11. By Southern blot hybridization and/or polymerase chain reaction, biopsies from all patients were positive for HPV 6/11 DNA. There was no difference in antibody response between cases and controls. Female cases and controls had significantly higher antibody titers than male subjects. A correlation was observed between the HPV-specific antibody level and the number of surgery-necessitating recurrences.
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