Effect of the tripeptide glycyl-L-histidyl-L-lysine on the proliferation and synthetic activity of chick embryo chondrocytes
Jazyk angličtina Země Nizozemsko Médium print
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
8562779
DOI
10.1016/0142-9612(95)93115-t
PII: 014296129593115T
Knihovny.cz E-zdroje
- MeSH
- buněčné dělení účinky léků MeSH
- chrupavka cytologie účinky léků metabolismus MeSH
- denaturace proteinů MeSH
- DNA analýza metabolismus MeSH
- ELISA MeSH
- kolagen biosyntéza chemie MeSH
- kultivační média MeSH
- kultivované buňky MeSH
- kuřecí embryo MeSH
- kůže metabolismus MeSH
- molekulární sekvence - údaje MeSH
- oligopeptidy farmakologie MeSH
- plastické hmoty chemie metabolismus MeSH
- protézy a implantáty normy MeSH
- růstové látky farmakologie MeSH
- sekvence aminokyselin MeSH
- skot MeSH
- zvířata MeSH
- Check Tag
- kuřecí embryo MeSH
- skot MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- DNA MeSH
- glycyl-histidyl-lysine MeSH Prohlížeč
- kolagen MeSH
- kultivační média MeSH
- oligopeptidy MeSH
- plastické hmoty MeSH
- růstové látky MeSH
Under certain conditions chondrocytes form lattices with cartilage collagens, which may serve as cartilage implants. It is necessary to find the optimal conditions for culturing chondrocytes. Three different supports are compared: (a) plastic; (b) cartilage collagens; and (c) insoluble skin collagen solubilized under denaturing conditions (ISC-40). The effect of culture medium supplementation with the tripeptide (Gly-His-Lys)2.Cu.2H2O.2NaCl (GHK) on chondrocyte proliferation and synthetic activity is studied, with particular attention paid to collagen types I, II and III. The collagen supports stimulated chondrocyte proliferation, but on the ISC-40 support they started to dedifferentiate rather early. In the primary culture, chondrocytes on all three supports synthesized mainly collagen type II, and only small amounts of types I and III. In the first passage the synthesis of these two collagen types increased, relative to collagen type II, at least on the cartilage collagen support. Supplementation of culture medium with GHK stimulated chondrocyte proliferation in the primary structure mostly on the ISC-40 support. On the other two types of supports the stimulatory effect of GHK was expressed mostly in the first passages. The collagen synthetic rate was increased by GHK on both of the collagen supports; on the cartilage collagen support collagen type II was synthesized predominantly and on the ISC-40 support types I and III were mostly formed. It is suggested that supplementation of culture medium with GHK may be useful in the preparation of cartilage implants.
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