Effect of the tripeptide glycyl-L-histidyl-L-lysine on the proliferation and synthetic activity of chick embryo chondrocytes
Language English Country Netherlands Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
8562779
DOI
10.1016/0142-9612(95)93115-t
PII: 014296129593115T
Knihovny.cz E-resources
- MeSH
- Cell Division drug effects MeSH
- Cartilage cytology drug effects metabolism MeSH
- Protein Denaturation MeSH
- DNA analysis metabolism MeSH
- Enzyme-Linked Immunosorbent Assay MeSH
- Collagen biosynthesis chemistry MeSH
- Culture Media MeSH
- Cells, Cultured MeSH
- Chick Embryo MeSH
- Skin metabolism MeSH
- Molecular Sequence Data MeSH
- Oligopeptides pharmacology MeSH
- Plastics chemistry metabolism MeSH
- Prostheses and Implants standards MeSH
- Growth Substances pharmacology MeSH
- Amino Acid Sequence MeSH
- Cattle MeSH
- Animals MeSH
- Check Tag
- Chick Embryo MeSH
- Cattle MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- DNA MeSH
- glycyl-histidyl-lysine MeSH Browser
- Collagen MeSH
- Culture Media MeSH
- Oligopeptides MeSH
- Plastics MeSH
- Growth Substances MeSH
Under certain conditions chondrocytes form lattices with cartilage collagens, which may serve as cartilage implants. It is necessary to find the optimal conditions for culturing chondrocytes. Three different supports are compared: (a) plastic; (b) cartilage collagens; and (c) insoluble skin collagen solubilized under denaturing conditions (ISC-40). The effect of culture medium supplementation with the tripeptide (Gly-His-Lys)2.Cu.2H2O.2NaCl (GHK) on chondrocyte proliferation and synthetic activity is studied, with particular attention paid to collagen types I, II and III. The collagen supports stimulated chondrocyte proliferation, but on the ISC-40 support they started to dedifferentiate rather early. In the primary culture, chondrocytes on all three supports synthesized mainly collagen type II, and only small amounts of types I and III. In the first passage the synthesis of these two collagen types increased, relative to collagen type II, at least on the cartilage collagen support. Supplementation of culture medium with GHK stimulated chondrocyte proliferation in the primary structure mostly on the ISC-40 support. On the other two types of supports the stimulatory effect of GHK was expressed mostly in the first passages. The collagen synthetic rate was increased by GHK on both of the collagen supports; on the cartilage collagen support collagen type II was synthesized predominantly and on the ISC-40 support types I and III were mostly formed. It is suggested that supplementation of culture medium with GHK may be useful in the preparation of cartilage implants.
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