Verocytotoxin-producing Escherichia coli in children with hemolytic uremic syndrome in the Czech Republic
Language English Country Germany Media print
Document type Journal Article
PubMed
8832150
Knihovny.cz E-resources
- MeSH
- Bacterial Toxins biosynthesis immunology MeSH
- Cytotoxins biosynthesis immunology MeSH
- Child MeSH
- Enzyme-Linked Immunosorbent Assay MeSH
- Escherichia coli immunology isolation & purification metabolism MeSH
- Feces microbiology MeSH
- Hemolytic-Uremic Syndrome epidemiology immunology microbiology MeSH
- Immunoblotting MeSH
- Escherichia coli Infections epidemiology etiology immunology MeSH
- Infant MeSH
- Humans MeSH
- Adolescent MeSH
- Child, Preschool MeSH
- Prospective Studies MeSH
- Antibodies, Bacterial analysis MeSH
- Serologic Tests MeSH
- Shiga Toxin 1 MeSH
- Check Tag
- Child MeSH
- Infant MeSH
- Humans MeSH
- Adolescent MeSH
- Male MeSH
- Child, Preschool MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
- Geographicals
- Czech Republic epidemiology MeSH
- Names of Substances
- Bacterial Toxins MeSH
- Cytotoxins MeSH
- Antibodies, Bacterial MeSH
- Shiga Toxin 1 MeSH
Since October 1988 till July 1995, 35 children (mean age 16 +/- 15.5 months) with classical hemolytic uremic syndrome (HUS) were examined for the presence of verocytotoxin (VT)-producing Escherichia coli (VTEC) infection. Stool samples from all patients were cultured for VTEC strains and tested for free fecal neutralizable VT. Serum samples from 18 patients taken on admission were also tested for antibodies to the lipopolysaccharide (LPS) from E. coli O157, O26, O55, O111, and O128 using the passive hemagglutination assay (PHA). Diagnosis of VTEC infection was established in 28 (80%) patients by the combined use of microbiological and serological techniques. VTEC were isolated from 16 (46%) patients, 5 of them had infection with 2 different VTEC serotypes; 11 (52%) VTEC isolates belonged to the serotypes O26: H11 (5), and O157: H7/NM (6). Free fecal VT was found in 21 (60%) patients. PHA antibodies to one or more LPS were detected in 14 (78%) of 18 patients; 9 had antibodies to O157 LPS and 6 to O26 LPS. We conclude from this study that VTEC are the important cause of pediatric HUS in the Czech Republic and the strains belonging to the serogroups O157 and O26 are the most prevalent.
