Morphological features of bone healing under the effect of collagen-graft-glycosaminoglycan copolymer supplemented with the tripeptide Gly-His-Lys
Jazyk angličtina Země Nizozemsko Médium print
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
8842360
DOI
10.1016/0142-9612(95)00310-x
PII: 014296129500310X
Knihovny.cz E-zdroje
- MeSH
- 4-chinolony * MeSH
- absorpce MeSH
- antiinfekční látky MeSH
- biodegradace MeSH
- biokompatibilní materiály * MeSH
- buňky pojivové tkáně MeSH
- chinolony MeSH
- femur MeSH
- fluorochinolony * MeSH
- gely MeSH
- glykosaminoglykany MeSH
- hojení ran * MeSH
- kolagen * imunologie MeSH
- kosti a kostní tkáň * cytologie MeSH
- králíci MeSH
- krysa rodu Rattus MeSH
- kůže MeSH
- kyčelní protézy * MeSH
- miniaturní prasata MeSH
- oligopeptidy * MeSH
- orchiektomie MeSH
- osteogeneze MeSH
- pefloxacin MeSH
- polyurethany * MeSH
- prasata MeSH
- protézy a implantáty * MeSH
- tvorba protilátek MeSH
- zvířata MeSH
- Check Tag
- králíci MeSH
- krysa rodu Rattus MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- 4-chinolony * MeSH
- antiinfekční látky MeSH
- biokompatibilní materiály * MeSH
- chinolony MeSH
- fluorochinolony * MeSH
- gely MeSH
- glycyl-histidyl-lysine MeSH Prohlížeč
- glykosaminoglykany MeSH
- kolagen * MeSH
- oligopeptidy * MeSH
- polyurethany * MeSH
The authors prepared 7.5% and 12.5% collagen gel, and supplemented it with the tripeptide Gly-His-Lys (GHK), perfloxacine and hypersulphated glycosaminoglycan (HSGAG). By means of 125l marking, its absorption was followed from small polyurethane sponges placed under the skin of rats. The absorption of gel without HSGAG was found to be faster. Antibodies against collagen (type I, II and III) or collagen gel were generated either in rabbits or in minipigs, in which collagen gel was tested. Microbiological tests proved the sterility of the collagen gel. The collagen gel supplemented with GHK, pefloxacine and HSGAG was named Colladel, and was used in a model experiment in guinea-pigs for filling artificially created bone defects in diaphyses of femurs, and with cementless endoprostheses. The healing process was followed by means of RTG and NMR, and histologically. The slowest healing process was found in unfilled bone defects. Defects filled with Colladel without GHK healed substantially more quickly, and the most accelerated healing was connected with complete Colladel application. When Colladel was used with cementless endoprostheses, vivid osteogenic activity at the interface of trabecular bone and metal stem was detectable in the course of the experiment.
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