The minimum period between the addition of killer toxin K1 to sensitive yeast cells and the appearance of first cells stained with bromocresol purple indicating membrane damage, is approximately 20 min. The length of this lag phase depends strongly on toxin concentration, extending sharply at toxin levels lower than 60 lethal units (LU) per cell (about one-tenth of the toxin concentration necessary for saturating all surface receptors). As the binding of the toxin to the cell is virtually complete within 1 min, the rest of the lag phase reflects processes different from actual binding, e.g. combination of several toxin molecules to form a membrane ion channel or pore.

Institute of Microbiology Academy of Sciences of the Czech Republic Prague

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Effect of killer toxin K1 on yeast membrane potential reported by the diS-C3(3) probe reflects strain- and physiological state-dependent variations