Effect of killer toxin K1 on yeast membrane potential reported by the diS-C3(3) probe reflects strain- and physiological state-dependent variations
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
10664883
DOI
10.1007/bf02818548
Knihovny.cz E-resources
- MeSH
- Fluorescent Dyes metabolism MeSH
- Fungal Proteins pharmacology MeSH
- Carbocyanines metabolism MeSH
- Killer Factors, Yeast MeSH
- Membrane Potentials drug effects MeSH
- Mycotoxins pharmacology MeSH
- Nystatin pharmacology MeSH
- Saccharomyces cerevisiae physiology MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- 3,3'-dipropylthiacarbocyanine MeSH Browser
- Fluorescent Dyes MeSH
- Fungal Proteins MeSH
- K1 killer toxin MeSH Browser
- Carbocyanines MeSH
- Killer Factors, Yeast MeSH
- Mycotoxins MeSH
- Nystatin MeSH
The rate and extent of uptake of the fluorescent probe diS-C3(3) reporting on membrane potential in S. cerevisiae is affected by the strain under study, cell-growth phase, starvation and by the concentration of glucose both in the growth medium and in the monitored cell suspension under non-growth conditions. Killer toxin K1 brings about changes in membrane potential. In all types of cells tested, viz. in glucose-supplied stationary or exponential cells of the killer-sensitive strain S6/1 or a conventional strain RXII, or in glucose-free exponential cells of both strains, both active and heat-inactivated toxin slow down the potential-dependent uptake of diS-C3(3) into the cells. This may reflect "clogging" of pores in the cell wall that hinders, but does not prevent, probe passage to the plasma membrane and its equilibration. The clogging effect of heat-inactivated toxin is stronger than that exerted by active toxin. In susceptible cells, i.e. in exponential-phase glucose-supplied cells of the sensitive strain S6/1, this phase of probe uptake retardation is followed by an irreversible red shift in probe fluorescence maximum lambda max indicating damage to membrane integrity and cell permeabilization. A similar fast red shift in lambda max signifying lethal cell damage was found in heat-killed or nystatin-treated cells.
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