Influence of the parental virus strain on the virulence and immunogenicity of recombinant vaccinia viruses expressing HBV preS2-S protein or VZV glycoprotein I
Language English Country Netherlands Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
8879101
DOI
10.1016/0264-410x(96)00008-4
PII: 0264410X96000084
Knihovny.cz E-resources
- MeSH
- Cell Line MeSH
- Species Specificity MeSH
- Genetic Vectors genetics metabolism MeSH
- Hepatitis B Surface Antigens biosynthesis immunology MeSH
- Mice, Inbred ICR MeSH
- Mice MeSH
- Protein Precursors biosynthesis immunology MeSH
- Viral Envelope Proteins biosynthesis immunology MeSH
- Antibodies, Viral biosynthesis MeSH
- Vaccines, Synthetic immunology MeSH
- Virulence MeSH
- Vaccinia virus genetics immunology pathogenicity MeSH
- Herpesvirus 3, Human metabolism MeSH
- Animals MeSH
- Check Tag
- Mice MeSH
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- glycoprotein IV, varicella-zoster virus MeSH Browser
- Hepatitis B Surface Antigens MeSH
- presurface protein 2, hepatitis B surface antigen MeSH Browser
- Protein Precursors MeSH
- Viral Envelope Proteins MeSH
- Antibodies, Viral MeSH
- Vaccines, Synthetic MeSH
Five triple-plaque purified vaccinia virus (VV) lines generated from smallpox Sevac VARIE vaccine (strain Praha) and three VV virus lines similarly derived from Wyeth DRYVAX vaccine were used for preparation of recombinants expressing the hepatitis B virus preS2-S gene. The same five Praha-derived virus lines were used to construct recombinants expressing the varicella-zoster virus (VZV) glycoprotein I (gpI) gene. Recombinants and their parental viruses were tested for the residual neurovirulence in mice. The virus lines and the recombinants derived therefrom differed markedly in this respect. Immunization of mice resulted in high levels of anti-HBsAg antibodies only in the case of recombinants derived from the relatively virulent viruses. In contrast, the levels of VZVgpI antibodies in mice were similar with all VV-VZV recombinants irrespective of the virulence of the parental virus line.
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