Nuclear transplantation in mammals: remodelling of transplanted nuclei under the influence of maturation promoting factor
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S., Review
PubMed
8885721
DOI
10.1002/bies.950181010
Knihovny.cz E-resources
- MeSH
- Cell Nucleus drug effects ultrastructure MeSH
- Cell Cycle MeSH
- Cytoplasm drug effects ultrastructure MeSH
- Microscopy, Electron MeSH
- Embryonic and Fetal Development MeSH
- Maturation-Promoting Factor pharmacology MeSH
- Oocytes drug effects ultrastructure MeSH
- Mammals MeSH
- Nuclear Transfer Techniques * MeSH
- Animals MeSH
- Check Tag
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Review MeSH
- Research Support, U.S. Gov't, Non-P.H.S. MeSH
- Names of Substances
- Maturation-Promoting Factor MeSH
Whilst the role of Maturation or M-phase Promoting Factor (MPF) as a universal M-phase regulator is well documented, much less attention has been paid to its role in nuclear transplantation experiments and especially to its influence upon remodelling of transplanted nuclei. There is currently wide acceptance that successful nuclear transplantation using differentiated nuclei is possible only in a cytoplasmic environment that is capable of inducing rapid nuclear de-differentiation to a pronuclear-like form. In this review our purpose is firstly, to outline the conditions under which such remodelling can be induced, and secondly, to extend the debate to include a consideration of whether complete nuclear remodelling is an absolute necessity for clonal development.
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