In vitro effects of arachidonic and L-glutamic acids on the high-affinity choline transport in rat hippocampus
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
9021765
DOI
10.1023/a:1027381421950
Knihovny.cz E-zdroje
- MeSH
- biologický transport účinky léků MeSH
- cholin farmakokinetika MeSH
- hemicholinium 3 metabolismus MeSH
- hipokampus cytologie účinky léků metabolismus MeSH
- krysa rodu Rattus MeSH
- kyselina arachidonová metabolismus farmakologie MeSH
- kyselina glutamová farmakologie MeSH
- lineární modely MeSH
- neurony účinky léků metabolismus MeSH
- potkani Wistar MeSH
- techniky in vitro MeSH
- vazebná místa MeSH
- zvířata MeSH
- Check Tag
- krysa rodu Rattus MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- cholin MeSH
- hemicholinium 3 MeSH
- kyselina arachidonová MeSH
- kyselina glutamová MeSH
A second messenger role for arachidonic acid (AA) in the regulation of the high-affinity choline uptake (HACU) was suggested. It was reported that micromolar concentrations of AA applied in vitro decreased the HACU values and increased the specific binding of [3H]hemicholinium-3 ([3H]HCh-3). It was published that L-glutamic acid (GA) applied in vivo produced a fall in the HACU values. In addition, GA liberates free AA. In this study, an ability of GA to influence in vitro the activity of presynaptic cholinergic nerve terminals via its effect on the release of AA is investigated in hippocampal synaptosomes of young Wistar rats. Millimolar concentrations of GA decrease both the high- and low-affinity choline uptake, the specific as well as nonspecific binding of [3H]HCh-3 and the activity of Na+, K(+)-ATPase. Kinetic analysis (Lineweaver-Burk and Scatchard plots) reveals a change in Vmax and Bmax, but not in KM and KD. It appears very likely that under normal conditions GA applied in vitro is not able to change markedly the choline transport via its effect on the release of AA. Results confirm the hypothesis about an indirect inhibitory role for glutamatergic receptors on cholinergic cells.
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