In vitro effects of arachidonic and L-glutamic acids on the high-affinity choline transport in rat hippocampus
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Biological Transport drug effects MeSH
- Choline pharmacokinetics MeSH
- Hemicholinium 3 metabolism MeSH
- Hippocampus cytology drug effects metabolism MeSH
- Rats MeSH
- Arachidonic Acid metabolism pharmacology MeSH
- Glutamic Acid pharmacology MeSH
- Linear Models MeSH
- Neurons drug effects metabolism MeSH
- Rats, Wistar MeSH
- In Vitro Techniques MeSH
- Binding Sites MeSH
- Animals MeSH
- Check Tag
- Rats MeSH
- Male MeSH
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Choline MeSH
- Hemicholinium 3 MeSH
- Arachidonic Acid MeSH
- Glutamic Acid MeSH
A second messenger role for arachidonic acid (AA) in the regulation of the high-affinity choline uptake (HACU) was suggested. It was reported that micromolar concentrations of AA applied in vitro decreased the HACU values and increased the specific binding of [3H]hemicholinium-3 ([3H]HCh-3). It was published that L-glutamic acid (GA) applied in vivo produced a fall in the HACU values. In addition, GA liberates free AA. In this study, an ability of GA to influence in vitro the activity of presynaptic cholinergic nerve terminals via its effect on the release of AA is investigated in hippocampal synaptosomes of young Wistar rats. Millimolar concentrations of GA decrease both the high- and low-affinity choline uptake, the specific as well as nonspecific binding of [3H]HCh-3 and the activity of Na+, K(+)-ATPase. Kinetic analysis (Lineweaver-Burk and Scatchard plots) reveals a change in Vmax and Bmax, but not in KM and KD. It appears very likely that under normal conditions GA applied in vitro is not able to change markedly the choline transport via its effect on the release of AA. Results confirm the hypothesis about an indirect inhibitory role for glutamatergic receptors on cholinergic cells.
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