Accumulation of Ade+ reversions in isoauxotrophic stains of Saccharomyces cerevisiae allelic in RAD6 during adenine starvation
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
9161002
Knihovny.cz E-resources
- MeSH
- Adenine metabolism MeSH
- Alleles MeSH
- Genes, Fungal MeSH
- Ligases genetics MeSH
- Mutation MeSH
- DNA Repair MeSH
- Saccharomyces cerevisiae Proteins * MeSH
- Saccharomyces cerevisiae genetics metabolism MeSH
- Ubiquitin-Conjugating Enzymes MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Adenine MeSH
- Ligases MeSH
- RAD6 protein, S cerevisiae MeSH Browser
- Saccharomyces cerevisiae Proteins * MeSH
- Ubiquitin-Conjugating Enzymes MeSH
A comparative method based on an analysis of accumulation of starvation-induced Ade+ reversions and cell death during adenine starvation was developed and exploited for estimating the role of RAD6 in the starvation-induced reversions. It was shown that inactivation of RAD6 function in Saccharomyces cerevisiae markedly enhances the accumulation of Ade+ reversions, and therefore it is likely that this gene is taking part in maintaining the low level of starvation-induced mutations in yeast cells.
