Dipeptidyl peptidase IV in C6 rat glioma cell line differentiation
Jazyk angličtina Země Německo Médium print
Typ dokumentu časopisecké články, práce podpořená grantem
- MeSH
- buněčná diferenciace fyziologie MeSH
- buněčná membrána enzymologie MeSH
- buněčné dělení fyziologie MeSH
- cytosol enzymologie MeSH
- dipeptidylpeptidasa 4 chemie metabolismus MeSH
- gliom enzymologie MeSH
- izoenzymy chemie metabolismus MeSH
- koncentrace vodíkových iontů MeSH
- krysa rodu Rattus MeSH
- kumariny metabolismus MeSH
- molekulová hmotnost MeSH
- nádorové buňky kultivované MeSH
- upregulace fyziologie MeSH
- zvířata MeSH
- Check Tag
- krysa rodu Rattus MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- dipeptidylpeptidasa 4 MeSH
- izoenzymy MeSH
- kumariny MeSH
The C6 rat glioma cell line is broadly used as a model in studies of glial cell differentiation. In the present study we demonstrated a significantly higher total cellular, but especially membrane-associated, activity of dipeptidyl peptidase IV in differentiated C6 cells in comparison with their proliferating counterparts. The majority, but not all, of enzyme isoelectric focusing isoforms from differentiated C6 cells displayed a substantially higher activity compared to the proliferating cells, with G-P-NHMec as the substrate. Non-denaturing polyacrylamide gradient gel electrophoresis showed the presence of one major peak of activity, dipeptidyl peptidase IV (Mr of about 220000), in both proliferating and differentiated C6 cells. The results indicate that dipeptidyl peptidase IV regulation is associated with C6 rat glioma cell differentiation.
Citace poskytuje Crossref.org
