Dipeptidyl peptidase IV in C6 rat glioma cell line differentiation
Language English Country Germany Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Cell Differentiation physiology MeSH
- Cell Membrane enzymology MeSH
- Cell Division physiology MeSH
- Cytosol enzymology MeSH
- Dipeptidyl Peptidase 4 chemistry metabolism MeSH
- Glioma enzymology MeSH
- Isoenzymes chemistry metabolism MeSH
- Hydrogen-Ion Concentration MeSH
- Rats MeSH
- Coumarins metabolism MeSH
- Molecular Weight MeSH
- Tumor Cells, Cultured MeSH
- Up-Regulation physiology MeSH
- Animals MeSH
- Check Tag
- Rats MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Dipeptidyl Peptidase 4 MeSH
- Isoenzymes MeSH
- Coumarins MeSH
The C6 rat glioma cell line is broadly used as a model in studies of glial cell differentiation. In the present study we demonstrated a significantly higher total cellular, but especially membrane-associated, activity of dipeptidyl peptidase IV in differentiated C6 cells in comparison with their proliferating counterparts. The majority, but not all, of enzyme isoelectric focusing isoforms from differentiated C6 cells displayed a substantially higher activity compared to the proliferating cells, with G-P-NHMec as the substrate. Non-denaturing polyacrylamide gradient gel electrophoresis showed the presence of one major peak of activity, dipeptidyl peptidase IV (Mr of about 220000), in both proliferating and differentiated C6 cells. The results indicate that dipeptidyl peptidase IV regulation is associated with C6 rat glioma cell differentiation.
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