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PubMed

Záznam pochází z PubMed

Combining Charge-Switch Derivatization with Ozone-Induced Dissociation for Fatty Acid Analysis

Poad, Berwyck L J
Autor Poad, Berwyck L J ORCID Central Analytical Research Facility, Queensland University of Technology, Brisbane, Australia. berwyck.poad@qut.edu.au
Marshall, David L
Autor Marshall, David L ORCID Central Analytical Research Facility, Queensland University of Technology, Brisbane, Australia
Harazim, Eva
Autor Harazim, Eva Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, Prague, Czech Republic
Gupta, Rajesh
Autor Gupta, Rajesh ORCID Central Analytical Research Facility, Queensland University of Technology, Brisbane, Australia
Narreddula, Venkateswara R
Autor Narreddula, Venkateswara R ORCID School of Chemistry, Physics and Mechanical Engineering, Queensland University of Technology, Brisbane, Australia
Young, Reuben S E
Autor Young, Reuben S E ORCID School of Chemistry, Physics and Mechanical Engineering, Queensland University of Technology, Brisbane, Australia
Duchoslav, Eva
Autor Duchoslav, Eva SCIEX, Concord, ON, Canada
Campbell, J Larry
Autor Campbell, J Larry ORCID SCIEX, Concord, ON, Canada
Broadbent, James A
Autor Broadbent, James A ORCID SCIEX, Concord, ON, Canada CSIRO Agriculture and Food, St Lucia, Queensland, 4067, Australia
Cvačka, Josef
Autor Autorita ORCID Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, Prague, Czech Republic
Mitchell, Todd W
Autor Mitchell, Todd W ORCID School of Medicine and Molecular Horizons, University of Wollongong, Wollongong, Australia
Blanksby, Stephen J
Autor Blanksby, Stephen J ORCID Central Analytical Research Facility, Queensland University of Technology, Brisbane, Australia. stephen.blanksby@qut.edu.au

Journal of the American Society for Mass Spectrometry. 2019 Oct ; 30 (10) : 2135-2143. [epub] 20190725

J Am Soc Mass Spectrom
ISSN 1879-1123 | 1044-0305
Zdroj

Jazyk angličtina Země Spojené státy americké Médium print-electronic

Typ dokumentu časopisecké články

Perzistentní odkaz https://www.medvik.cz/link/pmid31347025

Grantová podpora
DP150101715 Australian Research Council
DP190101486 Australian Research Council

PubMed 31347025
DOI 10.1007/s13361-019-02285-5
PII: 10.1007/s13361-019-02285-5
Knihovny.cz E-zdroje

Klíčová slova
Fatty Acids, Lipids, ozone-induced dissociation, vernix caseosa,
MeSH
lidé MeSH
mastné kyseliny analýza chemie MeSH
ozon chemie MeSH
plynová chromatografie s hmotnostně spektrometrickou detekcí MeSH
triglyceridy analýza chemie MeSH
vernix caseosa chemie MeSH
Check Tag
lidé MeSH
Publikační typ
časopisecké články MeSH
Názvy látek
mastné kyseliny MeSH
ozon MeSH
triglyceridy MeSH

The specific positions of carbon-carbon double bond(s) within an unsaturated fatty acid exert a significant effect on the physical and chemical properties of the lipid that ultimately inform its biological function(s). Contemporary liquid chromatography-mass spectrometry (MS) strategies based on electrospray ionization coupled to tandem MS can easily detect fatty acyl lipids but generally cannot reveal those specific site(s) of unsaturation. Herein, we describe a novel and versatile workflow whereby fatty acids are first converted to fixed charge N-(4-aminomethylphenyl)pyridinium (AMPP) derivatives and subsequently subjected to ozone-induced dissociation (OzID) on a modified triple quadrupole mass spectrometer. The AMPP modification enhances the detection of fatty acids introduced by direct infusion. Fragmentation of the derivatized fatty acids also provides diagnostic fragment ions upon collision-induced dissociation that can be targeted in precursor ion scans to subsequently trigger OzID analyses in an automated data-dependent workflow. It is these OzID analyses that provide unambiguous assignment of carbon-carbon double bond locations in the AMPP-derivatized fatty acids. The performance of this analysis pipeline is assessed in profiling the patterns of unsaturation in fatty acids within the complex biological secretion vernix caseosa. This analysis uncovers significant isomeric diversity within the fatty acid pool of this sample, including a number of hitherto unreported double bond positional isomers that hint at the activity of potentially new metabolic pathways.

Central Analytical Research Facility Queensland University of Technology Brisbane Australia

CSIRO Agriculture and Food St Lucia Queensland 4067 Australia

Institute of Organic Chemistry and Biochemistry Academy of Sciences of the Czech Republic Prague Czech Republic

School of Chemistry Physics and Mechanical Engineering Queensland University of Technology Brisbane Australia

School of Medicine and Molecular Horizons University of Wollongong Wollongong Australia

SCIEX Concord ON Canada

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