Differences in p53 and Bcl-2 expression in relation to cell proliferation during the development of human embryos
Jazyk angličtina Země Anglie, Velká Británie Médium print
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
9850335
PubMed Central
PMC395624
DOI
10.1136/mp.51.3.131
Knihovny.cz E-zdroje
- MeSH
- buněčné dělení fyziologie MeSH
- centrální nervový systém embryologie metabolismus MeSH
- embryonální a fetální vývoj fyziologie MeSH
- imunoenzymatické techniky MeSH
- ledviny embryologie metabolismus MeSH
- lidé MeSH
- nádorový supresorový protein p53 metabolismus MeSH
- plíce embryologie metabolismus MeSH
- protoonkogenní proteiny c-bcl-2 metabolismus MeSH
- trávicí systém embryologie metabolismus MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- nádorový supresorový protein p53 MeSH
- protoonkogenní proteiny c-bcl-2 MeSH
AIMS: To study the patterns of p53 and Bcl-2 expression in relation to cell proliferation during human embryogenesis in order to help elucidate their potential roles in the regulation of cell proliferation and apoptosis during morphogenesis. METHODS: Immunohistochemistry for p53, Bcl-2, and proliferating cell nuclear cell antigen (PCNA) proteins was performed, using a variety of monoclonal antibodies, on paraffin was embedded sections of tissues from 68 human embryos and fetuses of between 4 and 30 weeks gestation. RESULTS: Positive relations between sites of proliferative activity (as detected by PCNA expression) and p53 expression were found in the kidney, early developmental stages of intestine and lungs, liver, pancreas, heart, and in embryonic osteoblasts. On the other hand, positive relations between proliferative activity and Bcl-2 expression were found in the gonads, adrenal glands, in the cells of the dental lamina, hair follicles, syncytiotrophoblast, chondrocytes, and more advanced stages of intestinal development. In tissues of the central nervous system, p53 and Bcl-2 were co-expressed at the same sites but there was an inverse relation between p53/Bcl-2 expression and proliferative activity. CONCLUSIONS: These data suggest that p53 and Bcl-2 have tissue specific and stage specific functions during embryogenesis.
Zobrazit více v PubMed
Nature. 1992 Mar 19;356(6366):215-21 PubMed
Cancer Res. 1991 Dec 1;51(23 Pt 1):6304-11 PubMed
Cell. 1992 Jun 26;69(7):1237-45 PubMed
J Immunol Methods. 1992 Jul 6;151(1-2):237-44 PubMed
Development. 1991 Nov;113(3):857-65 PubMed
Cell. 1992 Aug 21;70(4):523-6 PubMed
Oncogene. 1993 Jan;8(1):203-7 PubMed
Oncogene. 1993 Feb;8(2):307-18 PubMed
Am J Pathol. 1993 Mar;142(3):743-53 PubMed
Nature. 1993 Apr 29;362(6423):847-9 PubMed
Nature. 1993 Apr 29;362(6423):849-52 PubMed
Oncogene. 1993 Jun;8(6):1487-94 PubMed
Cell. 1993 Oct 22;75(2):241-51 PubMed
Am J Pathol. 1994 Jul;145(1):61-73 PubMed
Nat Genet. 1995 Jun;10(2):175-80 PubMed
Curr Biol. 1995 Aug 1;5(8):931-6 PubMed
Genes Dev. 1996 Apr 1;10(7):836-50 PubMed
Nat Med. 1996 May;2(5):577-80 PubMed
Mol Cell Biol. 1996 Sep;16(9):5178-85 PubMed
EMBO J. 1996 Nov 15;15(22):6178-88 PubMed
Oncogene. 1996 Dec 19;13(12):2575-87 PubMed
Science. 1997 Feb 21;275(5303):1129-32 PubMed
Science. 1997 Feb 21;275(5303):1132-6 PubMed
Curr Biol. 1997 Mar 1;7(3):R144-7 PubMed
Mol Cell Biol. 1985 Oct;5(10):2851-5 PubMed
Biochim Biophys Acta. 1988 Sep 7;950(3):395-402 PubMed
Science. 1990 Aug 31;249(4972):1046-9 PubMed
Science. 1990 Aug 31;249(4972):1049-51 PubMed
Proc Natl Acad Sci U S A. 1991 Jun 1;88(11):5006-10 PubMed
Oncogene. 1991 Sep;6(9):1699-703 PubMed
Lancet. 1992 May 16;339(8803):1231 PubMed
