Expression of the hMLH1 and hMSH2 proteins in normal tissues: relationship to cancer predisposition in hereditary non-polyposis colon cancer
Jazyk angličtina Země Německo Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
- MeSH
- adaptorové proteiny signální transdukční MeSH
- chybné párování bází MeSH
- dědičné nepolypózní kolorektální nádory genetika MeSH
- DNA vazebné proteiny genetika MeSH
- endometrium chemie MeSH
- exprese genu * MeSH
- genetická predispozice k nemoci * MeSH
- homolog 2 proteinu MutS MeSH
- imunohistochemie MeSH
- jaderné proteiny MeSH
- játra chemie MeSH
- ledviny chemie MeSH
- lidé MeSH
- messenger RNA analýza MeSH
- močový měchýř chemie MeSH
- MutL homolog 1 MeSH
- nádorové proteiny genetika MeSH
- oprava DNA MeSH
- ovarium chemie MeSH
- protoonkogenní proteiny genetika MeSH
- střeva chemie MeSH
- transportní proteiny MeSH
- ureter chemie MeSH
- žaludek chemie MeSH
- Check Tag
- lidé MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- adaptorové proteiny signální transdukční MeSH
- DNA vazebné proteiny MeSH
- homolog 2 proteinu MutS MeSH
- jaderné proteiny MeSH
- messenger RNA MeSH
- MLH1 protein, human MeSH Prohlížeč
- MSH2 protein, human MeSH Prohlížeč
- MutL homolog 1 MeSH
- nádorové proteiny MeSH
- protoonkogenní proteiny MeSH
- transportní proteiny MeSH
The majority of tumours in patients with hereditary non-polyposis colon cancer (HNPCC) occur in large intestine and endometrium; also, other tissues are at increased risk. We studied expression of hMLH1 and hMSH2 proteins in 148 normal samples of various tissues from non-HNPCC patients and in 14 normal colon tissues from HNPCC patients. Immunohistochemical technique was used. Intensity of nuclear staining, percentage of stained cells and H-scores were calculated. Tissues were divided into groups. Groups A, B and C included tissues with increased risk of cancer in HNPCC A) stomach, small and large bowel; (B) endometrium; (C) ovary, ureter, urinary bladder, kidney and liver. Group D tissues were without increased risk. Expression of the proteins was significantly higher in groups A, B and C compared with group D (P<0.0001, P=0.0004 for hMSH2 in C versus D). The expression was highest in testis. In colons of HNPCC patients, expression of the mutated gene product was significantly lower than in non-HNPCC patients. In conclusion, hMLH1/hMSH2 protein expression is constitutively higher in certain cell types of certain tissues, including the majority of tissues that are at increased risk of cancer in HNPCC. However, association of strong hMLH1/hMSH2 expression with cancer risk is not strictly valid.
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