Extracellular acidification by Saccharomyces cerevisiae in normal and in heavy water
Language English Country United States Media print
Document type Comparative Study, Journal Article, Research Support, Non-U.S. Gov't
PubMed
10069011
DOI
10.1007/bf02816379
Knihovny.cz E-resources
- MeSH
- Potassium Chloride pharmacology MeSH
- Hydrogen-Ion Concentration MeSH
- Acids metabolism MeSH
- Deuterium Oxide pharmacology MeSH
- Buffers MeSH
- Saccharomyces cerevisiae drug effects metabolism MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Comparative Study MeSH
- Names of Substances
- Potassium Chloride MeSH
- Acids MeSH
- Deuterium Oxide MeSH
- Buffers MeSH
Titratable acidity of the extracellular medium was compared with that calculated from pH changes in a suspension of Saccharomyces cerevisiae. After addition of cells to normal water the ratio of titratable acidity to the computed one was about 25, after addition of 50 mmol/L D-glucose it was about 13, after subsequent addition of K+ ions it was only 2. In heavy water the respective values were 30, 9, and 1. Apparently, the principal buffer-generating processes have to do with glucose metabolism but little with the K+/H+ exchange observed after addition of K+. D2O appears to block processes producing the buffering capacity of the medium, among them possibly extrusion of organic acids.
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