Pseudoazurin mediates periplasmic electron flow in a mutant strain of Paracoccus denitrificans lacking cytochrome c550
Language English Country England, Great Britain Media print
Document type Journal Article
PubMed
10217431
DOI
10.1016/s0014-5793(99)00345-2
PII: S0014-5793(99)00345-2
Knihovny.cz E-resources
- MeSH
- Azurin analogs & derivatives isolation & purification metabolism MeSH
- Cytochrome c Group physiology MeSH
- Copper metabolism MeSH
- Molecular Sequence Data MeSH
- Mutation * MeSH
- Paracoccus denitrificans genetics metabolism MeSH
- Periplasm metabolism MeSH
- Pseudomonas MeSH
- Amino Acid Sequence MeSH
- Electron Transport MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Azurin MeSH
- cytochrome C-550 MeSH Browser
- Cytochrome c Group MeSH
- Copper MeSH
- pseudoazurin MeSH Browser
A periplasmic protein able to transfer electrons from cytoplasmic membrane to the periplasmic nitrite reductase (cytochrome cd1) has been purified from the anoxically grown cytochrome c550 mutant strain Pd2121 and shown to be pseudoazurin by several independent criteria (molecular mass, copper content, visible spectrum, N-terminal amino acid sequence). Under our assay conditions, the half-saturation of electron transport occurred at about 10 microM pseudoazurin; the reaction was retarded by increasing ionic strength.
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