Complex genomic and phenotypic characterization of the related species Staphylococcus carnosus and Staphylococcus piscifermentans
Language English Country Great Britain, England Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Deoxyribonuclease EcoRI metabolism MeSH
- DNA, Bacterial chemistry genetics isolation & purification MeSH
- Species Specificity MeSH
- Phenotype MeSH
- Genotype MeSH
- Polymerase Chain Reaction methods MeSH
- Electrophoresis, Gel, Pulsed-Field MeSH
- Restriction Mapping MeSH
- DNA, Ribosomal genetics MeSH
- RNA, Ribosomal genetics MeSH
- Cluster Analysis MeSH
- Staphylococcus classification genetics MeSH
- Bacterial Typing Techniques * MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Deoxyribonuclease EcoRI MeSH
- DNA, Bacterial MeSH
- DNA, Ribosomal MeSH
- RNA, Ribosomal MeSH
On the basis of numerical analysis of 100 phenotypic features, the strains of two species, Staphylococcus carnosus and Staphylococcus piscifermentans, were differentiated into two separate phenons corresponding with the macrorestriction patterns of their genomic DNA, as well as with the results of ribotyping and PCR amplification of enterobacterial repetitive intergenic consensus sequences. One of the S. carnosus strains, the F-2 strain, was shown to be marginal, exhibiting the lowest genomic and phenotypic similarity to the S. carnosus type strain DSM 20501T. Two of the strains studied (strains S. carnosus SK 06 and S. piscifermentans SK 05) were phenotypically convergent, forming a separate phenon. They were phenotypically similar, even though the genomic DNA of one of them was homologous with that of the S. carnosus type strain, whereas that of the other was homologous with the genomic DNA of the S. piscifermentans type strain. In such cases, fingerprinting methods (particularly macrorestriction analysis and ribotyping) served as important correctives, as they allow phenotypically convergent strains to be distinguished on the basis of their genomic profiles. The results of this paper support the proposal for the new species Staphylococcus condimenti as well as the new subspecies Staphylococcus carnosus subsp. utilis.
References provided by Crossref.org
Identification of Staphylococcus piscifermentans from dog feces
