Immunohistochemical localization of laminin-1 in the acellular nerve grafts is associated with migrating Schwann cells which display corresponding integrin receptors
Jazyk angličtina Země Slovensko Médium print
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
10707839
Knihovny.cz E-zdroje
- MeSH
- antigeny CD29 metabolismus MeSH
- CD antigeny metabolismus MeSH
- imunohistochemie MeSH
- integrin alfa6 MeSH
- integriny metabolismus MeSH
- kolagen metabolismus MeSH
- kryoterapie MeSH
- krysa rodu Rattus MeSH
- laminin metabolismus MeSH
- nervus ischiadicus cytologie metabolismus transplantace MeSH
- Schwannovy buňky cytologie metabolismus MeSH
- zvířata MeSH
- Check Tag
- krysa rodu Rattus MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- antigeny CD29 MeSH
- CD antigeny MeSH
- integrin alfa6 MeSH
- integriny MeSH
- kolagen MeSH
- laminin 1 MeSH Prohlížeč
- laminin MeSH
The presence of laminin-1, collagen-IV, alpha6 and beta1 integrin chains was detected by indirect immunohistochemistry using biotin/streptavidin/HRP or gold-conjugated secondary antibody at the light and electron microscope level, respectively. Cryo-treated segment of the peripheral stump without living Schwann cells (S-100-) did not display immunoreactivity for laminin-1 and integrin's chains, while the migrating Schwann cells in the marginal regions were immunostained for the antigens. Isolated acellular nerve segments protected from migration of Schwann cells (S-100-) exhibited laminin-1-, beta1-, and alpha6- integrin chains immunoreactivities. Position of the basal lamina was verified by collagen-IV+ immunoreactivity. Results indicate that presence of the laminin in the peripheral nerve is related with living Schwann cells.
