Immunohistochemical localization of laminin-1 in the acellular nerve grafts is associated with migrating Schwann cells which display corresponding integrin receptors
Language English Country Slovakia Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
10707839
Knihovny.cz E-resources
- MeSH
- Integrin beta1 metabolism MeSH
- Antigens, CD metabolism MeSH
- Immunohistochemistry MeSH
- Integrin alpha6 MeSH
- Integrins metabolism MeSH
- Collagen metabolism MeSH
- Cryotherapy MeSH
- Rats MeSH
- Laminin metabolism MeSH
- Sciatic Nerve cytology metabolism transplantation MeSH
- Schwann Cells cytology metabolism MeSH
- Animals MeSH
- Check Tag
- Rats MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Integrin beta1 MeSH
- Antigens, CD MeSH
- Integrin alpha6 MeSH
- Integrins MeSH
- Collagen MeSH
- laminin 1 MeSH Browser
- Laminin MeSH
The presence of laminin-1, collagen-IV, alpha6 and beta1 integrin chains was detected by indirect immunohistochemistry using biotin/streptavidin/HRP or gold-conjugated secondary antibody at the light and electron microscope level, respectively. Cryo-treated segment of the peripheral stump without living Schwann cells (S-100-) did not display immunoreactivity for laminin-1 and integrin's chains, while the migrating Schwann cells in the marginal regions were immunostained for the antigens. Isolated acellular nerve segments protected from migration of Schwann cells (S-100-) exhibited laminin-1-, beta1-, and alpha6- integrin chains immunoreactivities. Position of the basal lamina was verified by collagen-IV+ immunoreactivity. Results indicate that presence of the laminin in the peripheral nerve is related with living Schwann cells.
