Processing of mRNA from human leukocytes by biomagnetical separation: comparison with current methods of RNA isolation
Language English Country Czech Republic Media print
Document type Comparative Study, Journal Article, Research Support, Non-U.S. Gov't, Review
PubMed
10743720
Knihovny.cz E-resources
- MeSH
- Bronchoalveolar Lavage Fluid cytology MeSH
- Leukocytes chemistry MeSH
- Humans MeSH
- Magnetics MeSH
- RNA, Messenger blood isolation & purification MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Review MeSH
- Comparative Study MeSH
- Names of Substances
- RNA, Messenger MeSH
The article refers to the technique of biomagnetical separation (BS) of mRNA using "Dynabeads mRNA DIRECT". After a brief survey of RNA isolation methods authors describe a general procedure of BS as well as its concrete application for mRNA isolation from bronchoalveolar cells. Authors report results of specific experiments to assess the efficiency and accuracy of mRNA isolation by BS. The applicability of BS technique for the gene expression studies is discussed in the end of the article.
