Protein overexport in a Saccharomyces cerevisiae mutant is not due to facilitated release of cell-surface proteins
Language English Country United States Media print
Document type Journal Article
PubMed
11271810
DOI
10.1007/bf02908954
Knihovny.cz E-resources
- MeSH
- Benzenesulfonates pharmacology MeSH
- Cell Wall chemistry metabolism MeSH
- Fungal Proteins metabolism MeSH
- Transcription, Genetic MeSH
- Hydrolases pharmacology MeSH
- Killer Factors, Yeast MeSH
- Membrane Proteins metabolism MeSH
- Mutation * MeSH
- Periplasm enzymology MeSH
- Proteins pharmacology MeSH
- Saccharomyces cerevisiae drug effects genetics metabolism MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Benzenesulfonates MeSH
- C.I. Fluorescent Brightening Agent 28 MeSH Browser
- Fungal Proteins MeSH
- Hydrolases MeSH
- Killer Factors, Yeast MeSH
- Membrane Proteins MeSH
- Proteins MeSH
- zymolyase MeSH Browser
Saccharomyces cerevisiae strain MW11 is a temperature-sensitive mutant which exports twenty times more proteins at 37 degrees C than parental or wild-type strains do. To understand the mechanism underlying the protein overexport in the mutant the possibility of an altered cell-wall structure leading to facilitated release of cell-surface proteins was studied. Data on calcofluor white and zymolyase sensitivities, resistance to killer 1 toxin and determination of exported acid phosphatase and invertase did not provide evidence for alterations in the cell-wall structure that could explain the protein overexport phenotype. The results were obtained in experiments when transcription of mutated gene was discontinued which permits the full expression of the protein overexport phenotype.
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