Different recognition of DNA modified by aatitumor cisplatin and its clinically ineffective trans isomer by tumor suppressor protein p53
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
11279186
DOI
10.1074/jbc.m101224200
PII: S0021-9258(19)32027-7
Knihovny.cz E-zdroje
- MeSH
- adukty DNA metabolismus MeSH
- buněčné linie MeSH
- cisplatina metabolismus MeSH
- isomerie MeSH
- konsenzuální sekvence MeSH
- lidé MeSH
- nádorový supresorový protein p53 metabolismus MeSH
- oligodeoxyribonukleotidy chemie metabolismus MeSH
- rekombinantní proteiny metabolismus MeSH
- sekvence nukleotidů MeSH
- Spodoptera MeSH
- substrátová specifita MeSH
- transfekce MeSH
- vazebná místa MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- adukty DNA MeSH
- cisplatin-DNA adduct MeSH Prohlížeč
- cisplatina MeSH
- nádorový supresorový protein p53 MeSH
- oligodeoxyribonukleotidy MeSH
- rekombinantní proteiny MeSH
- transplatin MeSH Prohlížeč
The p53 gene encodes a nuclear phosphoprotein that is biologically activated in response to genotoxic stresses including treatment with anticancer platinum drugs. The DNA binding activity of p53 protein is crucial for its tumor suppressor function. DNA interactions of active wild-type human p53 protein with DNA fragments and oligodeoxyribonucleotide duplexes modified by antitumor cisplatin and its clinically ineffective trans isomer (transplatin) were investigated by using a gel mobility shift assay. It was found that DNA adducts of cisplatin reduced binding affinity of the consensus DNA sequence to p53, whereas transplatin adducts did not. This result was interpreted to mean that the precise steric fit required for the formation and stability of the tetrameric complex of p53 with the consensus sequence cannot be attained, as a consequence of severe conformational perturbations induced in DNA by cisplatin adducts. The results also demonstrate an increase of the binding affinity of p53 to DNA lacking the consensus sequence and modified by cisplatin but not by transplatin. In addition, only major 1,2-GG intrastrand cross-links of cisplatin are responsible for this enhanced binding affinity of p53. The data base on structures of various DNA adducts of cisplatin and transplatin reveals distinctive structural features of 1,2-intrastrand cross-links of cisplatin, suggesting a unique role for this adduct in the binding of p53 to DNA lacking the consensus sequence. The results support the hypothesis that the mechanism of antitumor activity of cisplatin may also be associated with its efficiency to affect the binding affinity of platinated DNA to active p53 protein.
Citace poskytuje Crossref.org
Recognition of Local DNA Structures by p53 Protein
