Leucine and protein metabolism in rats with chronic renal insufficiency
Language English Country Germany Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
11370737
DOI
10.1078/0940-2993-00171
PII: S0940-2993(04)70011-1
Knihovny.cz E-resources
- MeSH
- Acidosis etiology metabolism MeSH
- Cholesterol blood MeSH
- Kidney Failure, Chronic metabolism pathology MeSH
- Isoleucine metabolism MeSH
- Muscle, Skeletal metabolism MeSH
- Creatinine blood MeSH
- Rats MeSH
- Leucine metabolism MeSH
- Urea blood MeSH
- Disease Models, Animal MeSH
- Nephrectomy MeSH
- Rats, Wistar MeSH
- Eating physiology MeSH
- Proteins metabolism MeSH
- Body Weight physiology MeSH
- Uremia etiology metabolism MeSH
- Valine metabolism MeSH
- Animals MeSH
- Check Tag
- Rats MeSH
- Male MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Cholesterol MeSH
- Isoleucine MeSH
- Creatinine MeSH
- Leucine MeSH
- Urea MeSH
- Proteins MeSH
- Valine MeSH
The aim of this study was to evaluate the effect of chronic uremia induced by 5/6 nephrectomy (5/6NX) on changes in protein and branched-chain amino acid (BCAA; valine, leucine and isoleucine) metabolism. The control group consisted of sham operated rats. Twenty eight weeks after surgery the parameters of protein and amino acid metabolism were evaluated using a primed constant intravenous infusion of L-[1-(14)C]leucine. A drop in BCAA levels and a significant increase in urea, creatinine and cholesterol were observed in plasma of all 5/6NX rats. However, severe uremia with acidosis developed only in one third of rats with 5/6NX. In 5/6NX rats with acidosis significant increases in proteolysis, leucine oxidation, leucine oxidized fraction, and leucine clearance were observed in comparison with the control group and rats with 5/6NX without acidosis. In addition, in 5/6NX rats with acidosis a significant decrease in valine concentration in gastrocnemius muscle was found. We conclude that marked activation of proteolysis occurs in severe chronic renal failure and is probably caused by metabolic changes related to acidosis development.
References provided by Crossref.org
Serine Metabolism in Health and Disease and as a Conditionally Essential Amino Acid
Muscle wasting in animal models of severe illness
Acute effects of acidosis on protein and amino acid metabolism in perfused rat liver
