Preparation of recombinant coat protein of Prunus necrotic ringspot virus
Language English Country Switzerland Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
11394580
Knihovny.cz E-resources
- MeSH
- Ilarvirus genetics isolation & purification MeSH
- Capsid genetics isolation & purification MeSH
- Cloning, Molecular MeSH
- Molecular Sequence Data MeSH
- Recombinant Proteins genetics isolation & purification MeSH
- Base Sequence MeSH
- Trees virology MeSH
- Genes, Viral MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Recombinant Proteins MeSH
The coat protein (CP) gene of Prunus necrotic ringspot virus (PNRSV) was cloned into pET 16b vector and expressed in Escherichia coli. CP-enriched fractions were prepared from whole cell lysate by differential centrifugation. The fraction sedimenting at 20,000 x g for 30 mins was used for preparation of a rabbit antiserum to CP. This antiserum had a titer of 1:2048 and reacted in a double-antibody sandwich ELISA (DAS-ELISA).
Production of polyclonal antibodies to a recombinant coat protein of potato virus Y
GENBANK
AF013287
