Production of polyclonal antibodies to a recombinant coat protein of potato virus Y
Language English Country United States Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Enzyme-Linked Immunosorbent Assay MeSH
- Rabbits MeSH
- Antibodies, Monoclonal biosynthesis MeSH
- Plant Diseases virology MeSH
- Potyvirus immunology MeSH
- Antibodies, Viral biosynthesis MeSH
- Recombinant Proteins immunology MeSH
- Solanum tuberosum virology MeSH
- Nicotiana virology MeSH
- Capsid Proteins immunology MeSH
- Blotting, Western MeSH
- Animals MeSH
- Check Tag
- Rabbits MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- coat protein, Potato virus Y MeSH Browser
- Antibodies, Monoclonal MeSH
- Antibodies, Viral MeSH
- Recombinant Proteins MeSH
- Capsid Proteins MeSH
The gene encoding the coat protein (CP) of a potato virus Y (PVY) was cloned into expression vector pMPM-A4Omega. PVY CP was expressed in Escherichia coli and the purified recombinant protein was used for raising rabbit polyclonal antibodies. The sera and antibodies were tested for the detection of PVY in the laboratory host Nicotiana tabacum cv. Petit Havana SR1 and in various cultivars of the natural host Solanum tuberosum by ELISA as well as by Western blots. The antibodies can be used for the detection of the whole strain spectrum of PVY by indirect plate trapped antigen ELISA and Western blot, but not by double antigen sandwich ELISA.
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