Polyclonal antibodies to a recombinant coat protein of Potato virus A
Language English Country Switzerland Media print
Document type Evaluation Study, Journal Article, Research Support, Non-U.S. Gov't
PubMed
12580376
Knihovny.cz E-resources
- MeSH
- Microscopy, Electron MeSH
- Enzyme-Linked Immunosorbent Assay MeSH
- Escherichia coli metabolism MeSH
- Cloning, Molecular MeSH
- Antibodies, Monoclonal immunology MeSH
- Potyvirus genetics immunology MeSH
- Antibodies, Viral analysis immunology MeSH
- Recombinant Proteins chemistry immunology MeSH
- Solanum tuberosum virology MeSH
- Capsid Proteins genetics immunology MeSH
- Blotting, Western MeSH
- Publication type
- Journal Article MeSH
- Evaluation Study MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Antibodies, Monoclonal MeSH
- Antibodies, Viral MeSH
- Recombinant Proteins MeSH
- Capsid Proteins MeSH
Specific mouse antibodies against a recombinant coat protein (CP) of Potato virus A (PVA) were produced. The PVA CP gene was cloned in an expression vector pMPM4omega. After expression in Escherichia coli the presence of the expressed CP was proved by Western blot analysis using polyclonal and monoclonal antibodies (MAbs). The expressed CP was purified by centrifugation in CsCl density gradient or on a sucrose cushion. The production of virus-like particles (VLPs) was proved by electron microscopy. The purified CP was used for preparation of a mouse antiserum which had a titer of 1:1024 in ELISA and reacted specifically in Western blot analysis and indirect plate-trapped antigen ELISA (PTA-ELISA).
Production of polyclonal antibodies to a recombinant coat protein of potato virus Y
