Polyclonal antibodies to a recombinant coat protein of Potato virus A
Jazyk angličtina Země Švýcarsko Médium print
Typ dokumentu hodnotící studie, časopisecké články, práce podpořená grantem
PubMed
12580376
Knihovny.cz E-zdroje
- MeSH
- elektronová mikroskopie MeSH
- ELISA MeSH
- Escherichia coli metabolismus MeSH
- klonování DNA MeSH
- monoklonální protilátky imunologie MeSH
- Potyvirus genetika imunologie MeSH
- protilátky virové analýza imunologie MeSH
- rekombinantní proteiny chemie imunologie MeSH
- Solanum tuberosum virologie MeSH
- virové plášťové proteiny genetika imunologie MeSH
- western blotting MeSH
- Publikační typ
- časopisecké články MeSH
- hodnotící studie MeSH
- práce podpořená grantem MeSH
- Názvy látek
- monoklonální protilátky MeSH
- protilátky virové MeSH
- rekombinantní proteiny MeSH
- virové plášťové proteiny MeSH
Specific mouse antibodies against a recombinant coat protein (CP) of Potato virus A (PVA) were produced. The PVA CP gene was cloned in an expression vector pMPM4omega. After expression in Escherichia coli the presence of the expressed CP was proved by Western blot analysis using polyclonal and monoclonal antibodies (MAbs). The expressed CP was purified by centrifugation in CsCl density gradient or on a sucrose cushion. The production of virus-like particles (VLPs) was proved by electron microscopy. The purified CP was used for preparation of a mouse antiserum which had a titer of 1:1024 in ELISA and reacted specifically in Western blot analysis and indirect plate-trapped antigen ELISA (PTA-ELISA).
Production of polyclonal antibodies to a recombinant coat protein of potato virus Y
