The Zygosaccharomyces rouxii strain CBS732 contains only one copy of the HOG1 and the SOD2 genes
Language English Country Netherlands Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
11403849
DOI
10.1016/s0168-1656(01)00274-7
PII: S0168-1656(01)00274-7
Knihovny.cz E-resources
- MeSH
- Fungal Proteins genetics metabolism MeSH
- Gene Dosage MeSH
- Cations MeSH
- Cloning, Molecular MeSH
- Mitogen-Activated Protein Kinases genetics metabolism MeSH
- Molecular Sequence Data MeSH
- Mutation MeSH
- Sodium-Hydrogen Exchangers * MeSH
- Gene Expression Regulation, Fungal MeSH
- Saccharomyces cerevisiae Proteins * MeSH
- Saccharomyces cerevisiae genetics MeSH
- Amino Acid Sequence MeSH
- Sequence Analysis MeSH
- Sequence Homology, Amino Acid MeSH
- Carrier Proteins genetics metabolism MeSH
- Zygosaccharomyces genetics physiology MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Fungal Proteins MeSH
- HOG1 protein, S cerevisiae MeSH Browser
- Cations MeSH
- Mitogen-Activated Protein Kinases MeSH
- Sodium-Hydrogen Exchangers * MeSH
- Saccharomyces cerevisiae Proteins * MeSH
- SOD2 protein, Zygosaccharomyces rouxii MeSH Browser
- SOD22 protein, Zygosaccharomyces MeSH Browser
- Carrier Proteins MeSH
The osmotolerant yeast Zygosaccharomyces rouxii CBS732 contains only one copy of the ZrHOG1 and ZrSOD2-22 genes. Both genes were cloned and sequenced (Acc. Nos. AJ132606 and AJ252273, respectively) and their sequences were compared to homologous pairs of genes from Z. rouxii ATCC42981 (genes Z-HOG1, Z-HOG2, Z-SOD2, Z-SOD22). The CBS732 ZrHog1p is shorter than its ATCC42981 counterparts (380 aa residues vs. 407 and 420 aa, respectively) and is more similar to ATCC42981 Z-Hog2p than to Z-Hog1p. Also its promoter region corresponds to that one of Z-HOG2. The CBS732 ZrHOG1 promoter region is recognised by Saccharomyces cerevisiae, and the gene product (MAP kinase ZrHog1p) presence fully complements the osmosensitivity of a S. cerevisiae hog1 mutant strain. The CBS ZrSOD2-22 gene is highly similar to ATCC42981 Z-SOD2 but it contains also a segment of 15 aa residues specific for Z-SOD22. Z. rouxii ZrSod2-22 Na(+)/H(+) antiporter expressed in S. cerevisiae shows better activity toward toxic Na(+) and Li(+) cations than does S. cerevisiae's own Nha1 antiporter, and is efficient in improving the halotolerance of some S. cerevisiae wild types.
References provided by Crossref.org
Differences in osmotolerant and cell-wall properties of two Zygosaccharomyces rouxii strains
Exploration of yeast alkali metal cation/H+ antiporters: sequence and structure comparison
GENBANK
AJ132606, AJ252273
