The Zygosaccharomyces rouxii strain CBS732 contains only one copy of the HOG1 and the SOD2 genes
Jazyk angličtina Země Nizozemsko Médium print
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
11403849
DOI
10.1016/s0168-1656(01)00274-7
PII: S0168-1656(01)00274-7
Knihovny.cz E-zdroje
- MeSH
- fungální proteiny genetika metabolismus MeSH
- genová dávka MeSH
- kationty MeSH
- klonování DNA MeSH
- mitogenem aktivované proteinkinasy genetika metabolismus MeSH
- molekulární sekvence - údaje MeSH
- mutace MeSH
- Na(+)-H(+) antiport * MeSH
- regulace genové exprese u hub MeSH
- Saccharomyces cerevisiae - proteiny * MeSH
- Saccharomyces cerevisiae genetika MeSH
- sekvence aminokyselin MeSH
- sekvenční analýza MeSH
- sekvenční homologie aminokyselin MeSH
- transportní proteiny genetika metabolismus MeSH
- Zygosaccharomyces genetika fyziologie MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- fungální proteiny MeSH
- HOG1 protein, S cerevisiae MeSH Prohlížeč
- kationty MeSH
- mitogenem aktivované proteinkinasy MeSH
- Na(+)-H(+) antiport * MeSH
- Saccharomyces cerevisiae - proteiny * MeSH
- SOD2 protein, Zygosaccharomyces rouxii MeSH Prohlížeč
- SOD22 protein, Zygosaccharomyces MeSH Prohlížeč
- transportní proteiny MeSH
The osmotolerant yeast Zygosaccharomyces rouxii CBS732 contains only one copy of the ZrHOG1 and ZrSOD2-22 genes. Both genes were cloned and sequenced (Acc. Nos. AJ132606 and AJ252273, respectively) and their sequences were compared to homologous pairs of genes from Z. rouxii ATCC42981 (genes Z-HOG1, Z-HOG2, Z-SOD2, Z-SOD22). The CBS732 ZrHog1p is shorter than its ATCC42981 counterparts (380 aa residues vs. 407 and 420 aa, respectively) and is more similar to ATCC42981 Z-Hog2p than to Z-Hog1p. Also its promoter region corresponds to that one of Z-HOG2. The CBS732 ZrHOG1 promoter region is recognised by Saccharomyces cerevisiae, and the gene product (MAP kinase ZrHog1p) presence fully complements the osmosensitivity of a S. cerevisiae hog1 mutant strain. The CBS ZrSOD2-22 gene is highly similar to ATCC42981 Z-SOD2 but it contains also a segment of 15 aa residues specific for Z-SOD22. Z. rouxii ZrSod2-22 Na(+)/H(+) antiporter expressed in S. cerevisiae shows better activity toward toxic Na(+) and Li(+) cations than does S. cerevisiae's own Nha1 antiporter, and is efficient in improving the halotolerance of some S. cerevisiae wild types.
Citace poskytuje Crossref.org
Differences in osmotolerant and cell-wall properties of two Zygosaccharomyces rouxii strains
Exploration of yeast alkali metal cation/H+ antiporters: sequence and structure comparison
GENBANK
AJ132606, AJ252273
