The fission yeast ortholog of the coregulator SKIP interacts with the small subunit of U2AF
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
11414703
DOI
10.1006/bbrc.2001.5108
PII: S0006-291X(01)95108-3
Knihovny.cz E-resources
- MeSH
- Transcriptional Activation MeSH
- Cell Cycle MeSH
- Nuclear Proteins metabolism MeSH
- Molecular Sequence Data MeSH
- Ribonucleoproteins metabolism MeSH
- Saccharomyces cerevisiae metabolism MeSH
- Schizosaccharomyces metabolism MeSH
- Splicing Factor U2AF MeSH
- Two-Hybrid System Techniques MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Nuclear Proteins MeSH
- Ribonucleoproteins MeSH
- Splicing Factor U2AF MeSH
- U2AF2 protein, human MeSH Browser
The mode of action of transcriptional coregulators may involve the recruitment of spliceosome components. Using the two-hybrid screen, we examined the interaction partners of spSNW1, the S. pombe ortholog of the human coregulator SNW1/SKIP/NCoA-62, and found it to interact with the small subunit of the splicing factor U2AF (spU2AF23). The interaction involves the C-terminal parts of spU2AF23 and spSNW1. Tagged variants of both proteins were expressed in S. pombe and the interaction was proved by coprecipitation in nuclear extracts. This interaction would explain the finding of SKIP in nuclear speckles (Mintz, P. J., et al., EMBO J. 18, 4308-4320, 1999) and in reconstituted spliceosomes (Neubauer, G., et al., Nat. Genet. 20, 46-50, 1998). We deleted the spSNW1 gene in the diploid strain and demonstrated that spSNW1 is an essential gene in S. pombe.
References provided by Crossref.org
Truncating the spliceosomal 'rope protein' Prp45 results in Htz1 dependent phenotypes
Stress-induced expression of p53 target genes is insensitive to SNW1/SKIP downregulation
GENBANK
AL032824
