Unusual diversity in alpha-amanitin sensitivity of RNA polymerases in trichomonads
Language English Country Netherlands Media print
Document type Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.
Grant support
AI28798
NIAID NIH HHS - United States
PubMed
11420110
DOI
10.1016/s0166-6851(01)00294-8
PII: S0166-6851(01)00294-8
Knihovny.cz E-resources
- MeSH
- Amanitins pharmacology MeSH
- Transcription, Genetic MeSH
- Enzyme Inhibitors pharmacology MeSH
- Dicarboxylic Acids pharmacology MeSH
- Lysophosphatidylcholines pharmacology MeSH
- Organophosphorus Compounds pharmacology MeSH
- Cell Membrane Permeability drug effects MeSH
- RNA, Protozoan biosynthesis MeSH
- RNA Polymerase II antagonists & inhibitors metabolism MeSH
- Trichomonadida drug effects enzymology MeSH
- Trichomonas vaginalis drug effects enzymology MeSH
- Tritrichomonas foetus drug effects enzymology MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Research Support, U.S. Gov't, P.H.S. MeSH
- Names of Substances
- Amanitins MeSH
- Enzyme Inhibitors MeSH
- Dicarboxylic Acids MeSH
- Lysophosphatidylcholines MeSH
- Organophosphorus Compounds MeSH
- RNA, Protozoan MeSH
- RNA Polymerase II MeSH
- tagetitoxin MeSH Browser
Previous studies in the parasitic protist Trichomonas vaginalis have revealed that protein coding genes are transcribed by an alpha-amanitin-resistant RNA polymerase (RNAP) II. To investigate whether this unusual property is a general characteristic of trichomonads, we addressed the physiology of RNA synthesis in lysolecithin-permeabilized cells. Unlike in T. vaginalis, RNAP II in Tritrichomonas foetus was highly sensitive to the inhibitor alpha-amanitin. On the other hand, RNAP III, identified by its sensitivity to the specific inhibitor tagetitoxin, was found to be resistant to alpha-amanitin in Tritrichomonas foetus, but showed a typical intermediate sensitivity in T. vaginalis. Extension of this study to an additional seven trichomonad species confirmed this genera specific pattern of alpha-amanitin sensitivity and highlighted an unusual diversity in RNAPs among trichomonads, a closely related group of unicellular eukaryotes.
References provided by Crossref.org
Frataxin, a conserved mitochondrial protein, in the hydrogenosome of Trichomonas vaginalis
